Interphase FISH for Rapid Identification of a Down Syndrome Animal Model

Overview

Journal Cytogenet Cell Genet

Publisher Karger

Specialty Genetics

Date 1999 Nov 27

PMID 10575227

Citations 6

Authors

J Strovel

J Stamberg

P J Yarowsky

Affiliations

Soon will be listed here.

Abstract

Mouse models of Down syndrome (trisomy 21, DS) have been developed to study the consequences of triplication of regions syntenic to distal human chromosome 21. We report a technique for the identification of segmental trisomy 16 (Ts65Dn) and diploid progeny at any age using interphase fluorescence in situ hybridization (FISH) of uncultured tail fibroblasts. Our technique is faster and less expensive than blood karyotyping.

Citing Articles

Pharmacotherapy with fluoxetine restores functional connectivity from the dentate gyrus to field CA3 in the Ts65Dn mouse model of down syndrome.

Stagni F, Magistretti J, Guidi S, Ciani E, Mangano C, Calza L PLoS One. 2013; 8(4):e61689.

PMID: 23620781 PMC: 3631158. DOI: 10.1371/journal.pone.0061689.

Early pharmacotherapy with fluoxetine rescues dendritic pathology in the Ts65Dn mouse model of down syndrome.

Guidi S, Stagni F, Bianchi P, Ciani E, Ragazzi E, Trazzi S Brain Pathol. 2012; 23(2):129-43.

PMID: 22817700 PMC: 8028975. DOI: 10.1111/j.1750-3639.2012.00624.x.

Early pharmacotherapy restores neurogenesis and cognitive performance in the Ts65Dn mouse model for Down syndrome.

Bianchi P, Ciani E, Guidi S, Trazzi S, Felice D, Grossi G J Neurosci. 2010; 30(26):8769-79.

PMID: 20592198 PMC: 6632890. DOI: 10.1523/JNEUROSCI.0534-10.2010.

Widespread impairment of cell proliferation in the neonate Ts65Dn mouse, a model for Down syndrome.

Contestabile A, Fila T, Cappellini A, Bartesaghi R, Ciani E Cell Prolif. 2009; 42(2):171-81.

PMID: 19317805 PMC: 6760818. DOI: 10.1111/j.1365-2184.2009.00587.x.

Lithium restores neurogenesis in the subventricular zone of the Ts65Dn mouse, a model for Down syndrome.

Bianchi P, Ciani E, Contestabile A, Guidi S, Bartesaghi R Brain Pathol. 2009; 20(1):106-18.

PMID: 19298631 PMC: 8094672. DOI: 10.1111/j.1750-3639.2008.00246.x.