Posttreatment Follow-Up of Brucellosis by PCR Assay

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1999 Nov 24

PMID 10565954

Citations 14

Authors

P Morata

M I Queipo-Ortuno

J M Reguera

M A Garcia-Ordonez

C Pichardo

J D Colmenero

Affiliations

Soon will be listed here.

Abstract

In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28 (96.5%) were negative at the conclusion of the treatment. PCR was positive for the two patients who had relapses and negative for another four who had suspected but unconfirmed relapses. PCR was negative for 98.3% of the follow-up samples from those patients who had a favorable evolution. In conclusion, PCR appears to be a very useful technique, not only for the initial diagnosis of the disease, but also for posttreatment follow-up and the early detection of relapses.

Citing Articles

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