Membrane Difference in Peripheral Blood Lymphocytes from Patients with Chronic Lymphocytic Leukemia and Hodgkin's Disease

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Jun 1

PMID 1056037

Citations 4

Authors

U Mintz

L Sachs

Affiliations

Soon will be listed here.

Abstract

Lymphocytes were isolated from the peripheral blood of 21 normal persons and 66 patients with chronic lymphocytic leukemia (CLL), CLL in remission, Hodgkin's disease, Hodgkin's disease in remission, various other tumors, or cardiovascular diseases; The lymphocytes were studied for cap formation and agglutinability by concanavalin A, and for cell attachment to the surface of a petri dish. The frequency of cap formation was lowest in lymphocytes from patients with untreated Hodgkin's disease (2.1 plus or minus 0.8%), next lowest in lymphocytes from patients with CLL who were or were not under treatment (7,0 plus or minus 1;3%), and also low in Hodgkin's disease in remission (10.6 plus or minus 1.2%). The frequencies of cap formation by lymphocytes from patients with various other tumors (19.1 plus or minus 2.5%), with CLL in remission (24.0 plus or minus 0.9%), and with nonmalignant diseases (26.0 plus or minus 2.2%) were more similar to the frequency found in lymphocytes from normal persons (29.4 plus or minus 2.8%). Lymphocytes from all the patients, including those in remission, showed a higher degree of agglutinability by concanavalin A than lymphocytes from normal persons. Cell attachment to a petri dish was highest with CLL, next highest with CLL in remission, and low for normal persons and all the other patients. Lymphocytes from normal persons that consisted predominantly of thymus-derived cells gave similar results to isolated normal bone marrow-derived cells. The results indicate that there were different changes in the surface membrane of lymphocytes from patients with CLL, CLL in remission, Hodgkin's disease, and Hodgkin's disease in remission, and that the patients in clinical remission still showed abnormalities in their lymphocytes.

Citing Articles

Regulation of the induction of colonies in vitro by normal human lymphocytes.

Gerassi E, Sachs L Proc Natl Acad Sci U S A. 1976; 73(12):4546-50.

PMID: 1087416 PMC: 431536. DOI: 10.1073/pnas.73.12.4546.

Differences in the formation of normal T lymphocyte colonies by peripheral blood cells from patients with chronic lymphocytic leukemia and Hodgkin's disease.

Douer D, Sachs L Clin Exp Immunol. 1979; 38(3):514-22.

PMID: 317033 PMC: 1537927.

Correlation between movement of concanavalin A membrane receptors and cytolysis. A scanning electron microscopy study.

Lustig S, Ascher O, Fishman P, Djaldetti M, PLUZNIK D J Cell Biol. 1977; 75(2 Pt 1):388-97.

PMID: 122536 PMC: 2109941. DOI: 10.1083/jcb.75.2.388.

Antibodies to beta 2 microglobulin in the sera of patients with systemic lupus erythematosus.

Ooi B, Ooi Y, Pesce A, POLLAK V Immunology. 1977; 33(4):535-41.

PMID: 72726 PMC: 1445385.

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