Inactivation of the Tricarboxylic Acid Cycle Aconitase Gene from Streptomyces Viridochromogenes Tü494 Impairs Morphological and Physiological Differentiation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Nov 13

PMID 10559181

Citations 7

Authors

D Schwartz

S Kaspar

G Kienzlen

K Muschko

W Wohlleben

Affiliations

Soon will be listed here.

Abstract

The tricarboxylic acid (TCA) cycle aconitase gene acnA from Streptomyces viridochromogenes Tü494 was cloned and analyzed. AcnA catalyzes the isomerization of citrate to isocitrate in the TCA cycle, as indicated by the ability of acnA to complement the aconitase-deficient Escherichia coli mutant JRG3259. An acnA mutant was unable to develop aerial mycelium and to sporulate, resulting in a bald phenotype. Furthermore, the mutant did not produce the antibiotic phosphinothricin tripeptide, demonstrating that AcnA also affects physiological differentiation.

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