Transcriptional Control of the Low-temperature-inducible Des Gene, Encoding the Delta5 Desaturase of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Nov 13

PMID 10559169

Citations 29

Authors

P S Aguilar

P Lopez

D de Mendoza

Affiliations

Soon will be listed here.

Abstract

The Bacillus subtilis des gene encodes the cold-inducible Delta5 lipid desaturase involved in the formation of unsaturated fatty acids from saturated phospholipid precursors. Here, we describe the expression pattern of the des gene in response to a temperature downshift from 37 to 20 degrees C. We found that the synthesis of des mRNA is undetectable at 37 degrees C but dramatically induced upon the temperature downshift. Decay characteristics of the des transcript as well as the in vivo decay of B. subtilis bulk mRNA were investigated. The results showed that the stability of the des transcript as well as of bulk mRNA lasted substantially longer at 20 degrees C than at 37 degrees C. Functional expression of des at 37 degrees C was achieved by exchanging its promoter with the non-cold shock spac promoter. These data provide the first direct evidence that temperature-mediated control of transcription is the major mechanism regulating the mRNA levels of the B. subtilis desaturase. The present results also demonstrate that the only component of the desaturation system regulated by temperature is the desaturase enzyme.

Citing Articles

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References

Hurme R, Berndt K, Normark S, Rhen M . A proteinaceous gene regulatory thermometer in Salmonella. Cell. 1997; 90(1):55-64. DOI: 10.1016/s0092-8674(00)80313-x. View

Jiang W, Jones P, Inouye M . Chloramphenicol induces the transcription of the major cold shock gene of Escherichia coli, cspA. J Bacteriol. 1993; 175(18):5824-8. PMC: 206661. DOI: 10.1128/jb.175.18.5824-5828.1993. View

Sakamoto T, Bryant D . Temperature-regulated mRNA accumulation and stabilization for fatty acid desaturase genes in the cyanobacterium Synechococcus sp. strain PCC 7002. Mol Microbiol. 1997; 23(6):1281-92. DOI: 10.1046/j.1365-2958.1997.3071676.x. View

Henner D . Inducible expression of regulatory genes in Bacillus subtilis. Methods Enzymol. 1990; 185:223-8. DOI: 10.1016/0076-6879(90)85022-g. View

Los D, Ray M, Murata N . Differences in the control of the temperature-dependent expression of four genes for desaturases in Synechocystis sp. PCC 6803. Mol Microbiol. 1997; 25(6):1167-75. DOI: 10.1046/j.1365-2958.1997.5641912.x. View

Aguilar P, Cronan Jr J, de Mendoza D . A Bacillus subtilis gene induced by cold shock encodes a membrane phospholipid desaturase. J Bacteriol. 1998; 180(8):2194-200. PMC: 107148. DOI: 10.1128/JB.180.8.2194-2200.1998. View

Grau R, Gardiol D, Glikin G, de Mendoza D . DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis. Mol Microbiol. 1994; 11(5):933-41. DOI: 10.1111/j.1365-2958.1994.tb00372.x. View

English N, Palmer C, Alworth W, Kang L, Hughes V, Wolf C . Fatty acid signals in Bacillus megaterium are attenuated by cytochrome P-450-mediated hydroxylation. Biochem J. 1997; 327 ( Pt 2):363-8. PMC: 1218802. DOI: 10.1042/bj3270363. View

Strauch M, de Mendoza D, Hoch J . cis-unsaturated fatty acids specifically inhibit a signal-transducing protein kinase required for initiation of sporulation in Bacillus subtilis. Mol Microbiol. 1992; 6(20):2909-17. DOI: 10.1111/j.1365-2958.1992.tb01750.x. View

10.

Graumann P, Marahiel M . A superfamily of proteins that contain the cold-shock domain. Trends Biochem Sci. 1998; 23(8):286-90. DOI: 10.1016/s0968-0004(98)01255-9. View

11.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

12.

Raya R, Bardowski J, Andersen P, Ehrlich S, Chopin A . Multiple transcriptional control of the Lactococcus lactis trp operon. J Bacteriol. 1998; 180(12):3174-80. PMC: 107819. DOI: 10.1128/JB.180.12.3174-3180.1998. View

13.

Yung B, Kornberg A . Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli. Proc Natl Acad Sci U S A. 1988; 85(19):7202-5. PMC: 282152. DOI: 10.1073/pnas.85.19.7202. View

14.

Spizizen J . TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958; 44(10):1072-8. PMC: 528696. DOI: 10.1073/pnas.44.10.1072. View

15.

VanBogelen R, Neidhardt F . Ribosomes as sensors of heat and cold shock in Escherichia coli. Proc Natl Acad Sci U S A. 1990; 87(15):5589-93. PMC: 54372. DOI: 10.1073/pnas.87.15.5589. View

16.

Thieringer H, Jones P, Inouye M . Cold shock and adaptation. Bioessays. 1998; 20(1):49-57. DOI: 10.1002/(SICI)1521-1878(199801)20:1<49::AID-BIES8>3.0.CO;2-N. View

17.

Jaacks K, Healy J, Losick R, Grossman A . Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis. J Bacteriol. 1989; 171(8):4121-9. PMC: 210181. DOI: 10.1128/jb.171.8.4121-4129.1989. View

18.

Graumann P, Marahiel M . Some like it cold: response of microorganisms to cold shock. Arch Microbiol. 1996; 166(5):293-300. DOI: 10.1007/s002030050386. View

19.

Drider D, Santos J, Arraiano C, Lopez P . RNA processing is involved in the post-transcriptional control of the citQRP operon from Lactococcus lactis biovar diacetylactis. Mol Gen Genet. 1998; 258(1-2):9-15. DOI: 10.1007/s004380050701. View

20.

Fujii D, FULCO A . Biosynthesis of unsaturated fatty acids by bacilli. Hyperinduction and modulation of desaturase synthesis. J Biol Chem. 1977; 252(11):3660-70. View