Differentiation of Clonal Lines of Teratocarcinoma Cells: Formation of Embryoid Bodies in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Apr 1

PMID 1055416

Citations 178

Authors

G R Martin

M J Evans

Affiliations

Soon will be listed here.

Abstract

The differentiation in vitro of clonal pluripotent teratocarcinoma cells is reported. The first stage of this process is the formation of simple embryoid bodies which are identical to those found in animals bearing intraperitoneal teratocarcinomas. They consist of an inner core of embryonal carcinoma cells surrounded by a layer of endodermal cells which produce Reichert's membrane. The endodermal cells become apparent shortly after the embryonal carcinoma cells have formed aggregates which are loosely attached to the substratum. One clonal teratocarcinoma line was found to produce complex cystic embryoid bodies in vitro. Following formation of the endodermal cells, extensive differentiation to a wide variety of cell types occurs. There are similarities between the process of embryoid body formation and the early events of differentiation of the mouse embryo.

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References

Berstine E, Hooper M, Grandchamp S, EPHRUSSI B . Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973; 70(12):3899-903. PMC: 427353. DOI: 10.1073/pnas.70.12.3899. View

Gearhart J, Mintz B . Contact-mediated myogenesis and increased acetylcholinesterase activity in primary cultures of mouse teratocarcinoma cells. Proc Natl Acad Sci U S A. 1974; 71(5):1734-8. PMC: 388313. DOI: 10.1073/pnas.71.5.1734. View

KLEINSMITH L, Pierce Jr G . MULTIPOTENTIALITY OF SINGLE EMBRYONAL CARCINOMA CELLS. Cancer Res. 1964; 24:1544-51. View

LITTLEFIELD J . SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964; 145(3633):709-10. DOI: 10.1126/science.145.3633.709. View

Pierce Jr G, MIDGLEY Jr A, Ram J, FELDMAN J . Pariental yolk sac carcinoma: clue to the histogenesis of Riechert's membrane of the mouse embryo. Am J Pathol. 1962; 41:549-66. PMC: 1949615. View

Stevens L . Embryology of testicular teratomas in strain 129 mice. J Natl Cancer Inst. 1959; 23:1249-95. View

Pierce Jr G, DIXON Jr F, VERNEY E . Teratocarcinogenic and tissue-forming potentials of the cell types comprising neoplastic embryoid bodies. Lab Invest. 1960; 9:583-602. View

Pierce G, DIXON Jr F . Testicular teratomas. I. Demonstration of teratogenesis by metamorphosis of multipotential cells. Cancer. 1959; 12(3):573-83. DOI: 10.1002/1097-0142(195905/06)12:3<573::aid-cncr2820120316>3.0.co;2-m. View

Stevens L . Studies on transplantable testicular teratomas of strain 129 mice. J Natl Cancer Inst. 1958; 20(6):1257-75. DOI: 10.1093/jnci/20.6.1257. View

10.

Herbert M, Graham C . Cell determination and biochemical differentiation of the early mammalian embryo. Curr Top Dev Biol. 1974; 8:151-78. DOI: 10.1016/s0070-2153(08)60608-0. View

11.

Damjanov I, Solter D, SKREB N . Enzyme histochemistry of experimental embryo-derived teratocarcinomas. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1971; 76(4):249-56. DOI: 10.1007/BF00304029. View

12.

Martin G, Rubin H . Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts. Exp Cell Res. 1974; 85(2):319-33. DOI: 10.1016/0014-4827(74)90133-5. View

13.

WARE L, AXELRAD A . Inherited resistance to N- and B-tropic murine leukemia viruses in vitro: evidence that congenic mouse strains SIM and SIM.R differ at the Fv-1 locus. Virology. 1972; 50(2):339-48. DOI: 10.1016/0042-6822(72)90385-6. View

14.

Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F . Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc Natl Acad Sci U S A. 1973; 70(10):2988-92. PMC: 427153. DOI: 10.1073/pnas.70.10.2988. View

15.

Nieuwkoop P . The organization center of the amphibian embryo: its origin, spatial organization, and morphogenetic action. Adv Morphog. 1973; 10:1-39. DOI: 10.1016/b978-0-12-028610-2.50005-8. View

16.

Jakob H, Boon T, Gaillard J, Nicolas J, Jacob F . [Teratocarcinoma of the mouse: isolation, culture and properties of pluripotential cells]. Ann Microbiol (Paris). 1973; 124(3):269-82. View

17.

Teresky A, Marsden M, Kuff E, Levine A . Morphological criteria for the in vitro differentiation of embryoid bodies produced by a transplantable teratoma of mice. J Cell Physiol. 1974; 84(2):319-32. DOI: 10.1002/jcp.1040840218. View

18.

Levine A, Torosian M, Sarokhan A, Teresky A . Biochemical criteria for the in vitro differentiation of embryoid bodies produced by a transplantable teratoma of mice. The production of acetylcholine esterase and creatine phosphokinase by teratoma cells. J Cell Physiol. 1974; 84(2):311-7. DOI: 10.1002/jcp.1040840217. View

19.

Solter D, Biczysko W, Pienkowski M, KOPROWSKI H . Ultrastructure of mouse egg cylinders developed in vitro. Anat Rec. 1974; 180(2):263-79. DOI: 10.1002/ar.1091800202. View

20.

Martin G, Evans M . The morphology and growth of a pluripotent teratocarcinoma cell line and its derivatives in tissue culture. Cell. 1974; 2(3):163-72. DOI: 10.1016/0092-8674(74)90090-7. View