A Role for the Replication Proteins PCNA, RF-C, Polymerase Epsilon and Cdc45 in Transcriptional Silencing in Saccharomyces Cerevisiae

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1999 Nov 5

PMID 10545450

Citations 48

Authors

R T Kamakaka

J Rine

Affiliations

Soon will be listed here.

Abstract

Transcriptional silencing in the budding yeast Saccharomyces cerevisiae may be linked to DNA replication and cell cycle progression. In this study, we have surveyed the effect of 41 mutations in genes with a role in replication, the cell cycle, and DNA repair on silencing at HMR. Mutations in PCNA (POL30), RF-C (CDC44), polymerase epsilon (POL2, DPB2, DPB11), and CDC45 were found to restore silencing at a mutant HMR silencer allele that was still a chromosomal origin of replication. Replication timing experiments indicated that the mutant HMR locus was replicated late in S-phase, at the same time as wild-type HMR. Restoration of silencing by PCNA and CDC45 mutations required the origin recognition complex binding site of the HMR-E silencer. Several models for the precise role of these replication proteins in silencing are discussed.

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References

Fox C, Loo S, Rine J . The origin recognition complex, SIR1, and the S phase requirement for silencing. Science. 1997; 276(5318):1547-51. DOI: 10.1126/science.276.5318.1547. View

Friedman K, DILLER J, Ferguson B, Nyland S, Brewer B, FANGMAN W . Multiple determinants controlling activation of yeast replication origins late in S phase. Genes Dev. 1996; 10(13):1595-607. DOI: 10.1101/gad.10.13.1595. View

Rivin C, FANGMAN W . Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae. J Cell Biol. 1980; 85(1):108-15. PMC: 2110590. DOI: 10.1083/jcb.85.1.108. View

Maine G, Sinha P, Tye B . Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics. 1984; 106(3):365-85. PMC: 1224244. DOI: 10.1093/genetics/106.3.365. View

Miller A, Nasmyth K . Role of DNA replication in the repression of silent mating type loci in yeast. Nature. 1984; 312(5991):247-51. DOI: 10.1038/312247a0. View

Patterson M, Sclafani R, FANGMAN W, Rosamond J . Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae. Mol Cell Biol. 1986; 6(5):1590-8. PMC: 367685. DOI: 10.1128/mcb.6.5.1590-1598.1986. View

Brewer B, FANGMAN W . The localization of replication origins on ARS plasmids in S. cerevisiae. Cell. 1987; 51(3):463-71. DOI: 10.1016/0092-8674(87)90642-8. View

Kimmerly W, Buchman A, Kornberg R, Rine J . Roles of two DNA-binding factors in replication, segregation and transcriptional repression mediated by a yeast silencer. EMBO J. 1988; 7(7):2241-53. PMC: 454575. DOI: 10.1002/j.1460-2075.1988.tb03064.x. View

Reynolds A, McCarroll R, Newlon C, FANGMAN W . Time of replication of ARS elements along yeast chromosome III. Mol Cell Biol. 1989; 9(10):4488-94. PMC: 362533. DOI: 10.1128/mcb.9.10.4488-4494.1989. View

10.

AXELROD A, Rine J . A role for CDC7 in repression of transcription at the silent mating-type locus HMR in Saccharomyces cerevisiae. Mol Cell Biol. 1991; 11(2):1080-91. PMC: 359783. DOI: 10.1128/mcb.11.2.1080-1091.1991. View

11.

Gardner K, Rine J, Fox C . A region of the Sir1 protein dedicated to recognition of a silencer and required for interaction with the Orc1 protein in saccharomyces cerevisiae. Genetics. 1999; 151(1):31-44. PMC: 1460464. DOI: 10.1093/genetics/151.1.31. View

12.

Smith J, Caputo E, Boeke J . A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol. 1999; 19(4):3184-97. PMC: 84112. DOI: 10.1128/MCB.19.4.3184. View

13.

Aparicio O, Weinstein D, Bell S . Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell. 1997; 91(1):59-69. DOI: 10.1016/s0092-8674(01)80009-x. View

14.

Sherman F . Getting started with yeast. Methods Enzymol. 1991; 194:3-21. DOI: 10.1016/0076-6879(91)94004-v. View

15.

Ferguson B, Brewer B, Reynolds A, FANGMAN W . A yeast origin of replication is activated late in S phase. Cell. 1991; 65(3):507-15. DOI: 10.1016/0092-8674(91)90468-e. View

16.

Hennessy K, Lee A, Chen E, Botstein D . A group of interacting yeast DNA replication genes. Genes Dev. 1991; 5(6):958-69. DOI: 10.1101/gad.5.6.958. View

17.

Araki H, Hamatake R, Johnston L, Sugino A . DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991; 88(11):4601-5. PMC: 51713. DOI: 10.1073/pnas.88.11.4601. View

18.

Araki H, Hamatake R, Morrison A, Johnson A, Johnston L, Sugino A . Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae. Nucleic Acids Res. 1991; 19(18):4867-72. PMC: 328781. DOI: 10.1093/nar/19.18.4867. View

19.

Kitada K, Johnston L, Sugino T, Sugino A . Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae are suppressed by the DBF4 gene, which is required for the G1/S cell cycle transition. Genetics. 1992; 131(1):21-9. PMC: 1204955. DOI: 10.1093/genetics/131.1.21. View

20.

Budd M, Campbell J . DNA polymerases delta and epsilon are required for chromosomal replication in Saccharomyces cerevisiae. Mol Cell Biol. 1993; 13(1):496-505. PMC: 358929. DOI: 10.1128/mcb.13.1.496-505.1993. View