GFP-tagged Balancer Chromosomes for Drosophila Melanogaster

Overview

Journal Mech Dev

Publisher Elsevier

Specialties Biology
Cell Biology
Reproductive Medicine

Date 1999 Oct 27

PMID 10534621

Citations 33

Authors

D Casso

F A Ramirez-Weber

T B Kornberg

Affiliations

Soon will be listed here.

Abstract

We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae, and adults. We show the patterns of GFP expression of these balancers and demonstrate the use of the balancers to identify homozygous progeny.

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