The Homeodomain Transcription Factor CDP/cut Interacts with the Cell Cycle Regulatory Element of Histone H4 Genes Packaged into Nucleosomes

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 1999 Oct 26

PMID 10532314

Citations 8

Authors

T J Last

A J van Wijnen

M C de Ridder

G S Stein

J L Stein

Affiliations

Soon will be listed here.

Abstract

The homeodomain transcription factor CDP/cut contains four separate DNA binding domains and interacts with large segments of DNA. Thus, CDP/cut has the potential to function as an architectural protein and perhaps to support modifications in chromatin structure and nucleosomal organization. To begin to examine the ability of CDP/cut to interact with chromatin, we analyzed binding of CDP/cut to the histone H4 gene promoter (-90 to +75) reconstituted into nucleosome cores. The -90 to +75 region encompasses the cell cycle regulatory element (Site II) that controls histone H4 gene transcription, a CDP/cut binding site and a nuclease hypersensitive region. Using electrophoretic mobility shift assays and DNase I footprinting experiments, we show that CDP/cut specifically interacts with its recognition motif in a nucleosomal context without displacing the nucleosome core. The competency of CDP/cut to interact with nucleosomes suggests that this transcription factor may facilitate chromatin remodeling in response to cell cycle regulatory and/or developmental cues.

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