Spore Peptidoglycan Structure in a CwlD DacB Double Mutant of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Sep 28

PMID 10498740

Citations 10

Authors

D L Popham

C E Costello

P Setlow

Affiliations

Soon will be listed here.

Abstract

Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycine residues but no significant changes in spore resistance or core hydration.

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References

Popham D, Helin J, Costello C, Setlow P . Analysis of the peptidoglycan structure of Bacillus subtilis endospores. J Bacteriol. 1996; 178(22):6451-8. PMC: 178530. DOI: 10.1128/jb.178.22.6451-6458.1996. View

Ulijasz A, Grenader A, Weisblum B . A vancomycin-inducible lacZ reporter system in Bacillus subtilis: induction by antibiotics that inhibit cell wall synthesis and by lysozyme. J Bacteriol. 1996; 178(21):6305-9. PMC: 178505. DOI: 10.1128/jb.178.21.6305-6309.1996. View

Forrest T, Wilson G, Pan Y, Schaefer J . Characterization of cross-linking of cell walls of Bacillus subtilis by a combination of magic-angle spinning NMR and gas chromatography-mass spectrometry of both intact and hydrolyzed 13C- and 15N-labeled cell-wall peptidoglycan. J Biol Chem. 1991; 266(36):24485-91. View

Sekiguchi J, Akeo K, Yamamoto H, Khasanov F, Alonso J, Kuroda A . Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis. . J Bacteriol. 1995; 177(19):5582-9. PMC: 177368. DOI: 10.1128/jb.177.19.5582-5589.1995. View

Atrih A, Zollner P, Allmaier G, Foster S . Structural analysis of Bacillus subtilis 168 endospore peptidoglycan and its role during differentiation. J Bacteriol. 1996; 178(21):6173-83. PMC: 178487. DOI: 10.1128/jb.178.21.6173-6183.1996. View

Popham D, Helin J, Costello C, Setlow P . Muramic lactam in peptidoglycan of Bacillus subtilis spores is required for spore outgrowth but not for spore dehydration or heat resistance. Proc Natl Acad Sci U S A. 1996; 93(26):15405-10. PMC: 26417. DOI: 10.1073/pnas.93.26.15405. View

Murray T, Popham D, Pearson C, Hand A, Setlow P . Analysis of outgrowth of Bacillus subtilis spores lacking penicillin-binding protein 2a. J Bacteriol. 1998; 180(24):6493-502. PMC: 107750. DOI: 10.1128/JB.180.24.6493-6502.1998. View

Lindsay J, Beaman T, Gerhardt P . Protoplast water content of bacterial spores determined by buoyant density sedimentation. J Bacteriol. 1985; 163(2):735-7. PMC: 219183. DOI: 10.1128/jb.163.2.735-737.1985. View

Popham D, Illades-Aguiar B, Setlow P . The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration. J Bacteriol. 1995; 177(16):4721-9. PMC: 177238. DOI: 10.1128/jb.177.16.4721-4729.1995. View

10.

Schleifer K, Kandler O . Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972; 36(4):407-77. PMC: 408328. DOI: 10.1128/br.36.4.407-477.1972. View

11.

Popham D, Gilmore M, Setlow P . Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties. J Bacteriol. 1998; 181(1):126-32. PMC: 103540. DOI: 10.1128/JB.181.1.126-132.1999. View

12.

Warth A, Strominger J . Structure of the peptidoglycan from spores of Bacillus subtilis. Biochemistry. 1972; 11(8):1389-96. DOI: 10.1021/bi00758a010. View

13.

Popham D, Setlow P . The cortical peptidoglycan from spores of Bacillus megaterium and Bacillus subtilis is not highly cross-linked. J Bacteriol. 1993; 175(9):2767-9. PMC: 204583. DOI: 10.1128/jb.175.9.2767-2769.1993. View

14.

BUCHANAN C, Gustafson A . Mutagenesis and mapping of the gene for a sporulation-specific penicillin-binding protein in Bacillus subtilis. J Bacteriol. 1992; 174(16):5430-5. PMC: 206382. DOI: 10.1128/jb.174.16.5430-5435.1992. View

15.

Atrih A, Zollner P, Allmaier G, Williamson M, Foster S . Peptidoglycan structural dynamics during germination of Bacillus subtilis 168 endospores. J Bacteriol. 1998; 180(17):4603-12. PMC: 107474. DOI: 10.1128/JB.180.17.4603-4612.1998. View

16.

Warth A, Strominger J . Structure of the peptidoglycan of bacterial spores: occurrence of the lactam of muramic acid. Proc Natl Acad Sci U S A. 1969; 64(2):528-35. PMC: 223376. DOI: 10.1073/pnas.64.2.528. View

17.

Nakashio S, Gerhardt P . Protoplast dehydration correlated with heat resistance of bacterial spores. J Bacteriol. 1985; 162(2):571-8. PMC: 218886. DOI: 10.1128/jb.162.2.571-578.1985. View

18.

Beaman T, Gerhardt P . Heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation. Appl Environ Microbiol. 1986; 52(6):1242-6. PMC: 239215. DOI: 10.1128/aem.52.6.1242-1246.1986. View

19.

Lewis J, Snell N, BURR H . Water Permeability of Bacterial Spores and the Concept of a Contractile Cortex. Science. 1960; 132(3426):544-5. DOI: 10.1126/science.132.3426.544. View

20.

Costello C . Time, life ... and mass spectrometry. New techniques to address biological questions. Biophys Chem. 1998; 68(1-3):173-88. DOI: 10.1016/s0301-4622(97)00033-1. View