An Alternatively Spliced Surfactant Protein B MRNA in Normal Human Lung: Disease Implication

Overview

Journal Biochem J

Specialty Biochemistry

Date 1999 Sep 24

PMID 10493923

Citations 3

Authors

Z Lin

G Wang

D E deMello

J Floros

Affiliations

Soon will be listed here.

Abstract

We identified an alternatively-spliced surfactant protein B (SP-B) mRNA from normal human lung with a 12 nt deletion at the beginning of exon 8. This deletion causes a loss of four amino acids in the SP-B precursor protein. Sequence comparison of the 3' splice sites reveals only one difference in the frequency of U/C in the 11 predominantly-pyrimidine nucleotide tract, 73% for the normal and 45% for the alternatively-spliced SP-B mRNA (77-99% for the consensus sequence). Analysis of SP-B mRNA in lung indicates that the abundance of the alternatively-spliced form is very low and varies among individuals. Although the relative abundance of the deletion form of SP-B mRNA remains constant among normal lungs, it is found with relatively higher abundance in the lungs of some individuals with diseases such as congenital alveolar proteinosis, respiratory distress syndrome, bronchopulmonary dysplasia, alveolar capillary dysplasia and hypophosphatasia. This observation points to the possibility that the alternative splicing is a potential regulatory mechanism of SP-B and may play a role in the pathogenesis of disease under certain circumstances.

Citing Articles

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PMID: 30333828 PMC: 6175982. DOI: 10.3389/fimmu.2018.02256.

An intronic ABCA3 mutation that is responsible for respiratory disease.

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Surfactant protein B gene variations enhance susceptibility to squamous cell carcinoma of the lung in German patients.

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