CS5 Pilus Biosynthesis Genes from Enterotoxigenic Escherichia Coli O115:H40

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Sep 11

PMID 10482530

Citations 5

Authors

T G Duthy

L H Staendner

P A Manning

M W Heuzenroeder

Affiliations

Soon will be listed here.

Abstract

We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in the same direction as the major subunit and are flanked by a number of insertion sequence regions have been identified. T7 polymerase-mediated overexpression of the cloned csf ORFs confirmed protein sizes based on the DNA sequences that encode them. The expression of only the csf region in E. coli K-12 resulted in the hemagglutination of human erythrocytes and the cell surface expression of CS5 pili, suggesting that the cluster contains all necessary information for CS5 pilus biogenesis and function.

Citing Articles

Enterotoxigenic : intestinal pathogenesis mechanisms and colonization resistance by gut microbiota.

Zhang Y, Tan P, Zhao Y, Ma X Gut Microbes. 2022; 14(1):2055943.

PMID: 35358002 PMC: 8973357. DOI: 10.1080/19490976.2022.2055943.

Implication of lateral genetic transfer in the emergence of Aeromonas hydrophila isolates of epidemic outbreaks in channel catfish.

Hossain M, Waldbieser G, Sun D, Capps N, Hemstreet W, Carlisle K PLoS One. 2013; 8(11):e80943.

PMID: 24278351 PMC: 3835674. DOI: 10.1371/journal.pone.0080943.

Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek.

Nuccio S, Baumler A Microbiol Mol Biol Rev. 2007; 71(4):551-75.

PMID: 18063717 PMC: 2168650. DOI: 10.1128/MMBR.00014-07.

Genetic characterization and immunogenicity of coli surface antigen 4 from enterotoxigenic Escherichia coli when it is expressed in a Shigella live-vector strain.

Altboum Z, Levine M, Galen J, Barry E Infect Immun. 2003; 71(3):1352-60.

PMID: 12595452 PMC: 148885. DOI: 10.1128/IAI.71.3.1352-1360.2003.

Identification and characterization of assembly proteins of CS5 pili from enterotoxigenic Escherichia coli.

Duthy T, Manning P, Heuzenroeder M J Bacteriol. 2002; 184(4):1065-77.

PMID: 11807067 PMC: 134801. DOI: 10.1128/jb.184.4.1065-1077.2002.

References

Thomas L, McConnell M, Rowe B, Field A . The possession of three novel coli surface antigens by enterotoxigenic Escherichia coli strains positive for the putative colonization factor PCF8775. J Gen Microbiol. 1985; 131(9):2319-26. DOI: 10.1099/00221287-131-9-2319. View

Sakellaris H, Scott J . New tools in an old trade: CS1 pilus morphogenesis. Mol Microbiol. 1999; 30(4):681-7. DOI: 10.1046/j.1365-2958.1998.01088.x. View

Caron J, Coffield L, Scott J . A plasmid-encoded regulatory gene, rns, required for expression of the CS1 and CS2 adhesins of enterotoxigenic Escherichia coli. Proc Natl Acad Sci U S A. 1989; 86(3):963-7. PMC: 286599. DOI: 10.1073/pnas.86.3.963. View

Heuzenroeder M, Neal B, Thomas C, Halter R, Manning P . Characterization and molecular cloning of the PCF8775 CS5 antigen from an enterotoxigenic Escherichia coli 0115:H40 isolated in Central Australia. Mol Microbiol. 1989; 3(3):303-10. DOI: 10.1111/j.1365-2958.1989.tb00175.x. View

Jalajakumari M, Thomas C, Halter R, Manning P . Genes for biosynthesis and assembly of CS3 pili of CFA/II enterotoxigenic Escherichia coli: novel regulation of pilus production by bypassing an amber codon. Mol Microbiol. 1989; 3(12):1685-95. DOI: 10.1111/j.1365-2958.1989.tb00154.x. View

Caron J, Scott J . A rns-like regulatory gene for colonization factor antigen I (CFA/I) that controls expression of CFA/I pilin. Infect Immun. 1990; 58(4):874-8. PMC: 258554. DOI: 10.1128/iai.58.4.874-878.1990. View

McCarthy J, GUALERZI C . Translational control of prokaryotic gene expression. Trends Genet. 1990; 6(3):78-85. DOI: 10.1016/0168-9525(90)90098-q. View

Savelkoul P, Willshaw G, McConnell M, Smith H, Hamers A, van der Zeijst B . Expression of CFA/I fimbriae is positively regulated. Microb Pathog. 1990; 8(2):91-9. DOI: 10.1016/0882-4010(90)90073-y. View

Prere M, Chandler M, Fayet O . Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences. J Bacteriol. 1990; 172(7):4090-9. PMC: 213396. DOI: 10.1128/jb.172.7.4090-4099.1990. View

10.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

11.

Willshaw G, Smith H, McConnell M, Rowe B . Cloning of regulator genes controlling fimbrial production by enterotoxigenic Escherichia coli. FEMS Microbiol Lett. 1991; 66(2):125-9. DOI: 10.1016/0378-1097(91)90320-a. View

12.

de Haan L, Willshaw G, van der Zeijst B, Gaastra W . The nucleotide sequence of a regulatory gene present on a plasmid in an enterotoxigenic Escherichia coli strain of serotype O167:H5. FEMS Microbiol Lett. 1991; 83(3):341-6. DOI: 10.1111/j.1574-6968.1991.tb04487.x. View

13.

Clark C, Heuzenroeder M, Manning P . Colonization factor antigen CFA/IV (PCF8775) of human enterotoxigenic Escherichia coli: nucleotide sequence of the CS5 determinant. Infect Immun. 1992; 60(3):1254-7. PMC: 257624. DOI: 10.1128/iai.60.3.1254-1257.1992. View

14.

Scott J, Wakefield J, RUSSELL P, Orndorff P, Froehlich B . CooB is required for assembly but not transport of CS1 pilin. Mol Microbiol. 1992; 6(3):293-300. DOI: 10.1111/j.1365-2958.1992.tb01471.x. View

15.

Jordi B, Willshaw G, van der Zeijst B, Gaastra W . The complete nucleotide sequence of region 1 of the CFA/I fimbrial operon of human enterotoxigenic Escherichia coli. DNA Seq. 1992; 2(4):257-63. DOI: 10.3109/10425179209020811. View

16.

Dodson K, Jacob-Dubuisson F, Striker R, Hultgren S . Outer-membrane PapC molecular usher discriminately recognizes periplasmic chaperone-pilus subunit complexes. Proc Natl Acad Sci U S A. 1993; 90(8):3670-4. PMC: 46363. DOI: 10.1073/pnas.90.8.3670. View

17.

Rost B, Sander C . Prediction of protein secondary structure at better than 70% accuracy. J Mol Biol. 1993; 232(2):584-99. DOI: 10.1006/jmbi.1993.1413. View

18.

Rost B, Sander C . Improved prediction of protein secondary structure by use of sequence profiles and neural networks. Proc Natl Acad Sci U S A. 1993; 90(16):7558-62. PMC: 47181. DOI: 10.1073/pnas.90.16.7558. View

19.

Schifferli D, Alrutz M . Permissive linker insertion sites in the outer membrane protein of 987P fimbriae of Escherichia coli. J Bacteriol. 1994; 176(4):1099-110. PMC: 205162. DOI: 10.1128/jb.176.4.1099-1110.1994. View

20.

Rost B, Sander C, Schneider R . PHD--an automatic mail server for protein secondary structure prediction. Comput Appl Biosci. 1994; 10(1):53-60. DOI: 10.1093/bioinformatics/10.1.53. View