Regulation of Major Histocompatibility Complex Class II Antigens on Human Alveolar Macrophages by Granulocyte-macrophage Colony-stimulating Factor in the Presence of Glucocorticoids

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1999 Sep 1

PMID 10469240

Citations 7

Authors

J J Caulfield

M H Fernandez

A R Sousa

S J Lane

T H Lee

C M Hawrylowicz

Affiliations

Soon will be listed here.

Abstract

Alveolar macrophages (AM) present antigen poorly to CD4+ T cells and respond weakly to interferon-gamma (IFN-gamma) for up-regulation of major histocompatibility complex (MHC) class II and costimulatory molecule expression. In atopic asthma, however, AM exhibit enhanced antigen-presenting cell (APC) activity. Since granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in the airways of asthmatic patients, we have investigated its role in modulating the APC function of AM. The effects of glucocorticoids were also studied since earlier studies showed optimal induction of MHC antigens on monocytes by GM-CSF in their presence. GM-CSF in the presence, but not the absence, of dexamethasone enhanced the expression of HLA-DR, -DP and -DQ antigens by AM. However AM and monocytes differed in the optimal concentration of steroid required to mediate this effect (10-10 m and 10-7 m, respectively). Induction of MHC antigens was glucocorticoid specific and independent of IFN-gamma. These studies suggest the existence of an IFN-gamma-independent pathway of macrophage activation, which may be important in regulating APC function within the lung.

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References

Stampfli M, Wiley R, Neigh G, Gajewska B, Lei X, Snider D . GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice. J Clin Invest. 1998; 102(9):1704-14. PMC: 509118. DOI: 10.1172/JCI4160. View

Caulfield J, Hawrylowicz C, Kemeny D, Lee T . GM-CSF increases the ability of cultured macrophages to support autologous CD4+ T-cell proliferation in response to Dermatophagoides pteronyssinus and PPD antigen. Immunology. 1997; 92(1):123-30. PMC: 1363991. DOI: 10.1046/j.1365-2567.1997.00320.x. View

McCombs C, Michalski J, Westerfield B, Light R . Human alveolar macrophages suppress the proliferative response of peripheral blood lymphocytes. Chest. 1982; 82(3):266-71. DOI: 10.1378/chest.82.3.266. View

Mayernik D, Ul-Haq A, Rinehart J . Differentiation-associated alteration in human monocyte-macrophage accessory cell function. J Immunol. 1983; 130(5):2156-60. View

Toews G, Vial W, DUNN M, Guzzetta P, Nunez G, Stastny P . The accessory cell function of human alveolar macrophages in specific T cell proliferation. J Immunol. 1984; 132(1):181-6. View

Venet A, HANCE A, Saltini C, Robinson B, Crystal R . Enhanced alveolar macrophage-mediated antigen-induced T-lymphocyte proliferation in sarcoidosis. J Clin Invest. 1985; 75(1):293-301. PMC: 423439. DOI: 10.1172/JCI111688. View

Lipscomb M, Lyons C, Nunez G, Ball E, Stastny P, Vial W . Human alveolar macrophages: HLA-DR-positive macrophages that are poor stimulators of a primary mixed leukocyte reaction. J Immunol. 1986; 136(2):497-504. View

Kradin R, McCarthy K, Gifford J, Schneeberger E . Antigen-independent binding of T-cells by dendritic cells and alveolar macrophages in the rat. Am Rev Respir Dis. 1989; 139(1):207-11. DOI: 10.1164/ajrccm/139.1.207. View

Alvaro-Gracia J, Zvaifler N, Firestein G . Cytokines in chronic inflammatory arthritis. IV. Granulocyte/macrophage colony-stimulating factor-mediated induction of class II MHC antigen on human monocytes: a possible role in rheumatoid arthritis. J Exp Med. 1989; 170(3):865-75. PMC: 2189430. DOI: 10.1084/jem.170.3.865. View

10.

Howell C, Pujol J, Crea A, Davidson R, Gearing A, Godard P . Identification of an alveolar macrophage-derived activity in bronchial asthma that enhances leukotriene C4 generation by human eosinophils stimulated by ionophore A23187 as a granulocyte-macrophage colony-stimulating factor. Am Rev Respir Dis. 1989; 140(5):1340-7. DOI: 10.1164/ajrccm/140.5.1340. View

11.

Robinson D, Hamid Q, Ying S, Tsicopoulos A, Barkans J, Bentley A . Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. N Engl J Med. 1992; 326(5):298-304. DOI: 10.1056/NEJM199201303260504. View

12.

Sadeghi R, Hawrylowicz C, Chernajovsky Y, Feldmann M . Synergism of glucocorticoids with granulocyte macrophage colony stimulating factor (GM-CSF) but not interferon gamma (IFN-gamma) or interleukin-4 (IL-4) on induction of HLA class II expression on human monocytes. Cytokine. 1992; 4(4):287-97. DOI: 10.1016/1043-4666(92)90069-4. View

13.

Gant V, Cluzel M, Shakoor Z, Rees P, Lee T, Hamblin A . Alveolar macrophage accessory cell function in bronchial asthma. Am Rev Respir Dis. 1992; 146(4):900-4. DOI: 10.1164/ajrccm/146.4.900. View

14.

Holt P, Oliver J, Bilyk N, McMenamin C, McMenamin P, Kraal G . Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages. J Exp Med. 1993; 177(2):397-407. PMC: 2190916. DOI: 10.1084/jem.177.2.397. View

15.

Robinson D, Bentley A, Hartnell A, Kay A, Durham S . Activated memory T helper cells in bronchoalveolar lavage fluid from patients with atopic asthma: relation to asthma symptoms, lung function, and bronchial responsiveness. Thorax. 1993; 48(1):26-32. PMC: 464232. DOI: 10.1136/thx.48.1.26. View

16.

Bilyk N, Holt P . Inhibition of the immunosuppressive activity of resident pulmonary alveolar macrophages by granulocyte/macrophage colony-stimulating factor. J Exp Med. 1993; 177(6):1773-7. PMC: 2191032. DOI: 10.1084/jem.177.6.1773. View

17.

Sousa A, Poston R, Lane S, Nakhosteen J, Lee T . Detection of GM-CSF in asthmatic bronchial epithelium and decrease by inhaled corticosteroids. Am Rev Respir Dis. 1993; 147(6 Pt 1):1557-61. DOI: 10.1164/ajrccm/147.6_Pt_1.1557. View

18.

Sallusto F, Lanzavecchia A . Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J Exp Med. 1994; 179(4):1109-18. PMC: 2191432. DOI: 10.1084/jem.179.4.1109. View

19.

Vecchiarelli A, Dottorini M, Pietrella D, Monari C, Retini C, Todisco T . Role of human alveolar macrophages as antigen-presenting cells in Cryptococcus neoformans infection. Am J Respir Cell Mol Biol. 1994; 11(2):130-7. DOI: 10.1165/ajrcmb.11.2.8049074. View

20.

Poulter L, Janossy G, Power C, Sreenan S, Burke C . Immunological/physiological relationships in asthma: potential regulation by lung macrophages. Immunol Today. 1994; 15(6):258-61. DOI: 10.1016/0167-5699(94)90004-3. View