Random Amplification of Polymorphic DNA Fingerprinting of Trypansoma Evansi

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 1999 Aug 26

PMID 10461802

Citations 3

Authors

S H Basagoudanavar

J R Rao

R K Singh

G Butchaiah

Affiliations

Soon will be listed here.

Abstract

The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles.

Citing Articles

Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction.

Gunasekar K, Tewari A, Sreekumar C, Gupta S, Rao J Parasitol Res. 2008; 103(5):1075-81.

PMID: 18626662 DOI: 10.1007/s00436-008-1095-0.

Molecular profiles of Trypanosoma brucei, T. evansi and T. equiperdum stocks revealed by the random amplified polymorphic DNA method.

Lun Z, Li A, Chen X, Lu L, Zhu X Parasitol Res. 2004; 92(4):335-40.

PMID: 14727188 DOI: 10.1007/s00436-003-1054-8.

In vitro generation of cloned populations of Toxoplasma gondii.

Sreekumar C, Rao J, Mishra A, Ray D, Bansal G Parasitol Res. 2003; 90(6):489-92.

PMID: 12827508 DOI: 10.1007/s00436-003-0898-2.

References

Boid R . Isoenzyme characterisation of 15 stocks of Trypanosoma evansi isolated from camels in the Sudan. Trop Med Parasitol. 1988; 39(1):45-50. View

Waitumbi J, Murphy N . Inter- and intra-species differentiation of trypanosomes by genomic fingerprinting with arbitrary primers. Mol Biochem Parasitol. 1993; 58(1):181-5. DOI: 10.1016/0166-6851(93)90105-7. View

Borst P, Gibson W . Kinetoplast DNA of Trypanosoma evansi. Mol Biochem Parasitol. 1987; 23(1):31-8. DOI: 10.1016/0166-6851(87)90184-8. View

Watanapokasin Y, Tananyutthawongese C, Uthaisang W, Chansiri K, Boonmatit C, Sarataphan N . Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction. Vet Parasitol. 1998; 78(4):259-64. DOI: 10.1016/s0304-4017(98)00145-9. View

Macpherson J, Gajadhar A . Specific amplification of Sarcocystis cruzi DNA using a randomly primed polymerase chain reaction assay. Vet Parasitol. 1994; 55(4):267-77. DOI: 10.1016/0304-4017(94)90069-8. View

Bowditch B, Albright D, G Williams J, Braun M . Use of randomly amplified polymorphic DNA markers in comparative genome studies. Methods Enzymol. 1993; 224:294-309. DOI: 10.1016/0076-6879(93)24022-m. View

Waitumbi J, Murphy N, Peregrine A . Genotype and drug-resistance phenotype of Trypanosoma evansi isolated from camels in northern Kenya. Ann Trop Med Parasitol. 1994; 88(6):677-83. DOI: 10.1080/00034983.1994.11812921. View

Nei M, Li W . Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979; 76(10):5269-73. PMC: 413122. DOI: 10.1073/pnas.76.10.5269. View

Reddy Y, Rao J, Butchaiah G, Sharma B . Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay. Vet Parasitol. 1998; 79(4):315-23. DOI: 10.1016/s0304-4017(98)00176-9. View

10.

Waitumbi J, Young J . Electrophoretic karyotyping is a sensitive epidemiological tool for studying Trypanosoma evansi infections. Vet Parasitol. 1994; 52(1-2):47-56. DOI: 10.1016/0304-4017(94)90034-5. View

11.

Schaffer H, Sederoff R . Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981; 115(1):113-22. DOI: 10.1016/0003-2697(81)90533-9. View

12.

Welsh J, McClelland M . Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res. 1991; 19(19):5275-9. PMC: 328887. DOI: 10.1093/nar/19.19.5275. View