Identification and Characterization of a Negative Regulator of FtsZ Ring Formation in Bacillus Subtilis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Aug 18

PMID 10449747

Citations 114

Authors

P A Levin

I G Kurtser

A D Grossman

Affiliations

Soon will be listed here.

Abstract

During the bacterial cell cycle, the tubulin-like cell-division protein FtsZ polymerizes into a ring structure that establishes the location of the nascent division site. We have identified a regulator of FtsZ ring formation in Bacillus subtilis. This protein, EzrA, modulates the frequency and position of FtsZ ring formation. The loss of ezrA resulted in cells with multiple FtsZ rings located at polar as well as medial sites. Moreover, the critical concentration of FtsZ required for ring formation was lower in ezrA null mutants than in wild-type cells. EzrA was associated with the cell membrane and also colocalized with FtsZ to the nascent septal site. We propose that EzrA interacts either with FtsZ or with one of its binding partners to promote depolymerization.

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References

Berger B, Wilson D, Wolf E, Tonchev T, Milla M, Kim P . Predicting coiled coils by use of pairwise residue correlations. Proc Natl Acad Sci U S A. 1995; 92(18):8259-63. PMC: 41136. DOI: 10.1073/pnas.92.18.8259. View

Perego M, Spiegelman G, Hoch J . Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol. 1988; 2(6):689-99. DOI: 10.1111/j.1365-2958.1988.tb00079.x. View

Pogliano K, Harry E, Losick R . Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy. Mol Microbiol. 1995; 18(3):459-70. DOI: 10.1111/j.1365-2958.1995.mmi_18030459.x. View

Gimeno R, Espenshade P, Kaiser C . SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation. J Cell Biol. 1995; 131(2):325-38. PMC: 2199979. DOI: 10.1083/jcb.131.2.325. View

Lin D, Levin P, Grossman A . Bipolar localization of a chromosome partition protein in Bacillus subtilis. Proc Natl Acad Sci U S A. 1997; 94(9):4721-6. PMC: 20791. DOI: 10.1073/pnas.94.9.4721. View

STOFFEL W, Muller R, Binczek E, Hofmann K . Mouse apolipoprotein AI. cDNA-derived primary structure, gene organisation and complete nucleotide sequence. Biol Chem Hoppe Seyler. 1992; 373(4):187-93. DOI: 10.1515/bchm3.1992.373.1.187. View

Hale C, de Boer P . Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli. Cell. 1997; 88(2):175-85. DOI: 10.1016/s0092-8674(00)81838-3. View

McNally F, Okawa K, Iwamatsu A, Vale R . Katanin, the microtubule-severing ATPase, is concentrated at centrosomes. J Cell Sci. 1996; 109 ( Pt 3):561-7. DOI: 10.1242/jcs.109.3.561. View

Pogliano K, Hofmeister A, Losick R . Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1997; 179(10):3331-41. PMC: 179115. DOI: 10.1128/jb.179.10.3331-3341.1997. View

10.

RayChaudhuri D . ZipA is a MAP-Tau homolog and is essential for structural integrity of the cytokinetic FtsZ ring during bacterial cell division. EMBO J. 1999; 18(9):2372-83. PMC: 1171320. DOI: 10.1093/emboj/18.9.2372. View

11.

Chen J, Weiss D, Ghigo J, Beckwith J . Septal localization of FtsQ, an essential cell division protein in Escherichia coli. J Bacteriol. 1999; 181(2):521-30. PMC: 93406. DOI: 10.1128/JB.181.2.521-530.1999. View

12.

HEIM R, Tsien R . Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol. 1996; 6(2):178-82. DOI: 10.1016/s0960-9822(02)00450-5. View

13.

Lowe J, Amos L . Crystal structure of the bacterial cell-division protein FtsZ. Nature. 1998; 391(6663):203-6. DOI: 10.1038/34472. View

14.

Bi E, Lutkenhaus J . Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring. J Bacteriol. 1993; 175(4):1118-25. PMC: 193028. DOI: 10.1128/jb.175.4.1118-1125.1993. View

15.

Bramhill D, Thompson C . GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules. Proc Natl Acad Sci U S A. 1994; 91(13):5813-7. PMC: 44087. DOI: 10.1073/pnas.91.13.5813. View

16.

Beall B, Lutkenhaus J . FtsZ in Bacillus subtilis is required for vegetative septation and for asymmetric septation during sporulation. Genes Dev. 1991; 5(3):447-55. DOI: 10.1101/gad.5.3.447. View

17.

Nogales E, Wolf S, Downing K . Structure of the alpha beta tubulin dimer by electron crystallography. Nature. 1998; 391(6663):199-203. DOI: 10.1038/34465. View

18.

Marston A, Thomaides H, Edwards D, Sharpe M, Errington J . Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev. 1998; 12(21):3419-30. PMC: 317235. DOI: 10.1101/gad.12.21.3419. View

19.

Ward Jr J, Lutkenhaus J . Overproduction of FtsZ induces minicell formation in E. coli. Cell. 1985; 42(3):941-9. DOI: 10.1016/0092-8674(85)90290-9. View

20.

Harry E, Pogliano K, Losick R . Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1995; 177(12):3386-93. PMC: 177040. DOI: 10.1128/jb.177.12.3386-3393.1995. View