Structure-based Design of an RNA-binding Zinc Finger

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Aug 18

PMID 10449725

Citations 20

Authors

D J McColl

C D Honchell

A D Frankel

Affiliations

Soon will be listed here.

Abstract

A structure-based approach was used to design RNA-binding zinc fingers that recognize the HIV-1 Rev response element (RRE). An arginine-rich alpha-helix from HIV-1 Rev was engineered into the zinc finger framework, and the designed fingers were shown to bind specifically to the RRE with high affinity and in a zinc-dependent manner, and display cobalt absorption and CD spectra characteristic of properly folded fingers. The results indicate that a monomeric zinc finger can recognize a specific nucleic acid site and that the alpha-helix of a finger can be used to recognize the major groove of RNA as well as DNA. The RRE-binding zinc fingers demonstrate how structure-based approaches may be used in the design of potential RNA-binding therapeutics and provide a framework for selecting RNA-binding fingers with desired specifications.

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