The Arcelin-5 Gene of Phaseolus Vulgaris Directs High Seed-specific Expression in Transgenic Phaseolus Acutifolius and Arabidopsis Plants

Overview

Journal Plant Physiol

Specialty Physiology

Date 1999 Aug 12

PMID 10444093

Citations 14

Authors

A Goossens

W Dillen

J De Clercq

M Van Montagu

G Angenon

Affiliations

Soon will be listed here.

Abstract

The regulatory sequences of many genes encoding seed storage proteins have been used to drive seed-specific expression of a variety of proteins in transgenic plants. Because the levels at which these transgene-derived proteins accumulate are generally quite low, we investigated the utility of the arcelin-5 regulatory sequences in obtaining high seed-specific expression in transgenic plants. Arcelin-5 is an abundant seed protein found in some wild common bean (Phaseolus vulgaris L.) genotypes. Seeds of Arabidopsis and Tepary bean (Phaseolus acutifolius A. Gray) plants transformed with arcelin-5 gene constructs synthesized arcelin-5 to levels of 15% and 25% of the total protein content, respectively. To our knowledge, such high expression levels directed by a transgene have not been reported before. The transgenic plants also showed low plant-to-plant variation in arcelin expression. Complex transgene integration patterns, which often result in gene silencing effects, were not associated with reduced arcelin-5 expression. High transgene expression was the result of high mRNA steady-state levels and was restricted to seeds. This indicates that all requirements for high seed-specific expression are cis elements present in the cloned genomic arcelin-5 sequence and trans-acting factors that are available in Arabidopsis and Phaseolus spp., and thus probably in most dicotyledonous plants.

Citing Articles

Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis.

Zeng J, Zhang M, Hou L, Bai W, Yan X, Hou N J Exp Bot. 2019; 70(12):3139-3151.

PMID: 30970146 PMC: 6598071. DOI: 10.1093/jxb/erz162.

A vacuolar sorting receptor-independent sorting mechanism for storage vacuoles in soybean seeds.

Maruyama N, Matsuoka Y, Yokoyama K, Takagi K, Yamada T, Hasegawa H Sci Rep. 2018; 8(1):1108.

PMID: 29348620 PMC: 5773536. DOI: 10.1038/s41598-017-18697-w.

The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants.

Hnatuszko-Konka K, Luchniak P, Wiktorek-Smagur A, Gerszberg A, Kowalczyk T, Gatkowska J World J Microbiol Biotechnol. 2016; 32(7):113.

PMID: 27263008 PMC: 4893371. DOI: 10.1007/s11274-016-2070-z.

Seed-to-seed-to-seed growth and development of Arabidopsis in microgravity.

Link B, Busse J, Stankovic B Astrobiology. 2014; 14(10):866-75.

PMID: 25317938 PMC: 4201294. DOI: 10.1089/ast.2014.1184.

Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity.

Piron R, De Koker S, De Paepe A, Goossens J, Grooten J, Nauwynck H PLoS One. 2014; 9(3):e91386.

PMID: 24614617 PMC: 3948849. DOI: 10.1371/journal.pone.0091386.

References

Zheng Z, Sumi K, Tanaka K, Murai N . The Bean Seed Storage Protein [beta]-Phaseolin Is Synthesized, Processed, and Accumulated in the Vacuolar Type-II Protein Bodies of Transgenic Rice Endosperm. Plant Physiol. 1995; 109(3):777-786. PMC: 161377. DOI: 10.1104/pp.109.3.777. View

Ellis J, Shirsat A, Hepher A, Yarwood J, Gatehouse J, Croy R . Tissue-specific expression of a pea legumin gene in seeds of Nicotiana plumbaginifolia. Plant Mol Biol. 2013; 10(3):203-14. DOI: 10.1007/BF00027397. View

Sengupta-Gopalan C, Reichert N, Barker R, Hall T, Kemp J . Developmentally regulated expression of the bean beta-phaseolin gene in tobacco seed. Proc Natl Acad Sci U S A. 1985; 82(10):3320-4. PMC: 397767. DOI: 10.1073/pnas.82.10.3320. View

Breyne P, Van Montagu M, Gheysen G . The role of scaffold attachment regions in the structural and functional organization of plant chromatin. Transgenic Res. 1994; 3(3):195-202. DOI: 10.1007/BF01973987. View

Schroeder H, Gollasch S, Moore A, Tabe L, Craig S, Hardie D . Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.). Plant Physiol. 1995; 107(4):1233-1239. PMC: 157257. DOI: 10.1104/pp.107.4.1233. View

Barthels N, van der Lee F, Klap J, Goddijn O, Karimi M, Puzio P . Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures. Plant Cell. 1998; 9(12):2119-34. PMC: 157062. DOI: 10.1105/tpc.9.12.2119. View

Vancanneyt G, Schmidt R, Willmitzer L, Rocha-Sosa M . Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet. 1990; 220(2):245-50. DOI: 10.1007/BF00260489. View

Romero Andreas J, Yandell B, Bliss F . Bean arcelin : 1. Inheritance of a novel seed protein of Phaseolus vulgaris L. and its effect on seed composition. Theor Appl Genet. 2013; 72(1):123-8. DOI: 10.1007/BF00261467. View

Higgins T, Newbigin E, Spencer D, Llewellyn D, Craig S . The sequence of a pea vicilin gene and its expression in transgenic tobacco plants. Plant Mol Biol. 2013; 11(5):683-95. DOI: 10.1007/BF00017468. View

10.

Beachy R, Chen Z, Horsch R, Rogers S, Hoffmann N, Fraley R . Accumulation and assembly of soybean beta-conglycinin in seeds of transformed petunia plants. EMBO J. 1985; 4(12):3047-53. PMC: 554621. DOI: 10.1002/j.1460-2075.1985.tb04044.x. View

11.

Thomas T . Gene expression during plant embryogenesis and germination: an overview. Plant Cell. 1993; 5(10):1401-10. PMC: 160371. DOI: 10.1105/tpc.5.10.1401. View

12.

Guerche P, de Almeida E, Schwarztein M, Gander E, Krebbers E, Pelletier G . Expression of the 2S albumin from Bertholletia excelsa in Brassica napus. Mol Gen Genet. 1990; 221(3):306-14. DOI: 10.1007/BF00259393. View

13.

Molvig L, Tabe L, Eggum B, Moore A, Craig S, Spencer D . Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proc Natl Acad Sci U S A. 1997; 94(16):8393-8. PMC: 22931. DOI: 10.1073/pnas.94.16.8393. View

14.

Sturm A, Voelker T, Herman E, Chrispeels M . Correct glycosylation, Golgi-processing, and targeting to protein bodies of the vacuolar protein phytohemagglutinin in transgenic tobacco. Planta. 2013; 175(2):170-83. DOI: 10.1007/BF00392425. View

15.

Hobbs S, Warkentin T, Delong C . Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol Biol. 1993; 21(1):17-26. DOI: 10.1007/BF00039614. View

16.

Altenbach S, Pearson K, Meeker G, Staraci L, Sun S . Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Mol Biol. 1989; 13(5):513-22. DOI: 10.1007/BF00027311. View

17.

Bagga S, Adams H, Kemp J, Sengupta-Gopalan C . Accumulation of 15-Kilodalton Zein in Novel Protein Bodies in Transgenic Tobacco. Plant Physiol. 1995; 107(1):13-23. PMC: 161159. DOI: 10.1104/pp.107.1.13. View

18.

Hoffman L, Donaldson D, Bookland R, Rashka K, Herman E . Synthesis and protein body deposition of maize 15-kd zein in transgenic tobacco seeds. EMBO J. 1987; 6(11):3213-21. PMC: 553772. DOI: 10.1002/j.1460-2075.1987.tb02638.x. View

19.

Saalbach I, Pickardt T, Machemehl F, Saalbach G, Schieder O, Muntz K . A chimeric gene encoding the methionine-rich 2S albumin of the Brazil nut (Bertholletia excelsa H.B.K.) is stably expressed and inherited in transgenic grain legumes. Mol Gen Genet. 1994; 242(2):226-36. DOI: 10.1007/BF00391017. View

20.

Slightom J, Drong R, Klassy R, Hoffman L . Nucleotide sequences from phaseolin cDNA clones: the major storage proteins from Phaseolus vulgaris are encoded by two unique gene families. Nucleic Acids Res. 1985; 13(18):6483-98. PMC: 321972. DOI: 10.1093/nar/13.18.6483. View