Identification of Inhibitory Mutants of Cauliflower Mosaic Virus Movement Protein Function After Expression in Insect Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1999 Aug 10

PMID 10438886

Citations 8

Authors

C L Thomas

A J Maule

Affiliations

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Abstract

Cauliflower mosaic virus (CaMV) encodes a movement protein (MP) which forms tubules in vivo and mediates the translocation of virus particles through plasmodesmata. The relationship between CaMV MP structure and function, in isolation from the complete virus infection, was studied by using MP expression in insect cells. The study allowed the MP domains necessary for tubule formation to be identified and potential MP-MP interactions to be investigated by using double infections with recombinant baculoviruses. Two MP domains which interfered with the ability of the wild-type MP to form tubules were identified. These mutant domains appeared to act as competitive, rather than dominant negative, inhibitors.

Citing Articles

A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins.

Amari K, Boutant E, Hofmann C, Schmitt-Keichinger C, Fernandez-Calvino L, Didier P PLoS Pathog. 2010; 6(9):e1001119.

PMID: 20886105 PMC: 2944810. DOI: 10.1371/journal.ppat.1001119.

Caulimoviridae tubule-guided transport is dictated by movement protein properties.

Sanchez-Navarro J, Fajardo T, Zicca S, Pallas V, Stavolone L J Virol. 2010; 84(8):4109-12.

PMID: 20130061 PMC: 2849483. DOI: 10.1128/JVI.02543-09.

Cauliflower mosaic virus gene VI product N-terminus contains regions involved in resistance-breakage, self-association and interactions with movement protein.

Hapiak M, Li Y, Agama K, Swade S, Okenka G, Falk J Virus Res. 2008; 138(1-2):119-29.

PMID: 18851998 PMC: 2610446. DOI: 10.1016/j.virusres.2008.09.002.

The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures.

Wei T, Kikuchi A, Moriyasu Y, Suzuki N, Shimizu T, Hagiwara K J Virol. 2006; 80(17):8593-602.

PMID: 16912308 PMC: 1563882. DOI: 10.1128/JVI.00537-06.

A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement.

Stavolone L, Villani M, Leclerc D, Hohn T Proc Natl Acad Sci U S A. 2005; 102(17):6219-24.

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