Evidence for Tumor-host Cooperation in Regulating MMP-2 Expression in Human Colon Cancer

Overview

Journal Clin Exp Metastasis

Specialty Oncology

Date 1999 Aug 4

PMID 10432005

Citations 6

Authors

D L Ornstein

J Macnab

K H Cohn

Affiliations

Soon will be listed here.

Abstract

Matrix metalloproteinase 2 (MMP-2) facilitates tumor growth and metastasis in colon cancer. Although tumor cells may produce MMP-2, stromal cells, such as macrophages and fibroblasts, contribute significantly to MMP-2 synthesis in human tumors. We characterized four human colon cancer cell lines with differing biological behavior for MMP-2 expression. While the parent tumors from which the cell lines were derived all expressed MMP-2 mRNA, MMP-2 transcripts were detected in only one cell line, TF-17C, which is nontumorigenic in a nude mouse tumor model. TF-43C, which is tumorigenic and metastatic in the same tumor model, did not produce MMP-2, yet the tumors which arose from it after injection into nude mice did contain MMP-2 mRNA, suggesting a contribution from stromal cells. Co-culturing TF-43C with fibroblasts resulted in an increase in MMP-2 protein, whereas co-culturing with the nontumorigenic cell line TF-13Cm did not alter constitutive fibroblast MMP-2 secretion. Conditioned medium from TF-43C cells also stimulated fibroblast MMP-2 production. These data suggest that a soluble factor from TF-43C cells can stimulate fibroblast MMP-2 production and support the hypothesis that colon cancer cell interactions with stromal fibroblasts may be important determinants of tumor behavior in vivo.

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References

Kataoka H, DeCastro R, Zucker S, Biswas C . Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase. Cancer Res. 1993; 53(13):3154-8. View

Forster S, Talbot I, Clayton D, Critchley D . Tumour basement membrane laminin in adenocarcinoma of rectum: an immunohistochemical study of biological and clinical significance. Int J Cancer. 1986; 37(6):813-7. DOI: 10.1002/ijc.2910370603. View

Riley H, Macnab J, Farrell T, Cohn K . The expression of acylphosphatase is associated with the metastatic phenotype in human colorectal tumors. Carcinogenesis. 1998; 18(12):2453-5. DOI: 10.1093/carcin/18.12.2453. View

An Z, Wang X, Willmott N, Chander S, Tickle S, Docherty A . Conversion of highly malignant colon cancer from an aggressive to a controlled disease by oral administration of a metalloproteinase inhibitor. Clin Exp Metastasis. 1997; 15(2):184-95. DOI: 10.1023/a:1018461112732. View

Zeng Z, Huang Y, Cohen A, Guillem J . Prediction of colorectal cancer relapse and survival via tissue RNA levels of matrix metalloproteinase-9. J Clin Oncol. 1996; 14(12):3133-40. DOI: 10.1200/JCO.1996.14.12.3133. View

Watson S, Morris T, Parsons S, Steele R, Brown P . Therapeutic effect of the matrix metalloproteinase inhibitor, batimastat, in a human colorectal cancer ascites model. Br J Cancer. 1996; 74(9):1354-8. PMC: 2074768. DOI: 10.1038/bjc.1996.549. View

Okada A, Bellocq J, Rouyer N, Chenard M, Rio M, Chambon P . Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas. Proc Natl Acad Sci U S A. 1995; 92(7):2730-4. PMC: 42292. DOI: 10.1073/pnas.92.7.2730. View

Barsky S, Siegal G, JANNOTTA F, Liotta L . Loss of basement membrane components by invasive tumors but not by their benign counterparts. Lab Invest. 1983; 49(2):140-7. View

Pyke C, Ralfkiaer E, Tryggvason K, Dano K . Messenger RNA for two type IV collagenases is located in stromal cells in human colon cancer. Am J Pathol. 1993; 142(2):359-65. PMC: 1886712. View

10.

Shah V, Kumar S, Zirvi K . Metastasis of human colon tumor cells in vivo: correlation with the overexpression of plasminogen activators and 72 kDa gelatinase. In Vivo. 1994; 8(3):321-6. View

11.

Emmert-Buck M, ROTH M, Zhuang Z, Campo E, Rozhin J, Sloane B . Increased gelatinase A (MMP-2) and cathepsin B activity in invasive tumor regions of human colon cancer samples. Am J Pathol. 1994; 145(6):1285-90. PMC: 1887494. View

12.

Migita K, Eguchi K, Kawabe Y, Ichinose Y, Tsukada T, Aoyagi T . TNF-alpha-mediated expression of membrane-type matrix metalloproteinase in rheumatoid synovial fibroblasts. Immunology. 1996; 89(4):553-7. PMC: 1456590. DOI: 10.1046/j.1365-2567.1996.d01-789.x. View

13.

Swallow C, Murray M, Guillem J . Metastatic colorectal cancer cells induce matrix metalloproteinase release by human monocytes. Clin Exp Metastasis. 1996; 14(1):3-11. DOI: 10.1007/BF00157680. View

14.

Huhtala P, Chow L, Tryggvason K . Structure of the human type IV collagenase gene. J Biol Chem. 1990; 265(19):11077-82. View

15.

Sato H, Takino T, Okada Y, Cao J, Shinagawa A, Yamamoto E . A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature. 1994; 370(6484):61-5. DOI: 10.1038/370061a0. View

16.

Ishikawa T, Ichikawa Y, Mitsuhashi M, Momiyama N, Chishima T, Tanaka K . Matrilysin is associated with progression of colorectal tumor. Cancer Lett. 1996; 107(1):5-10. DOI: 10.1016/0304-3835(96)04336-4. View

17.

Loizidou M, Carpenter R, Laurie H, Cooper A, Alexander P, Taylor I . Growth enhancement of implanted human colorectal cancer cells by the addition of fibroblasts in vivo. Br J Surg. 1996; 83(1):24-8. DOI: 10.1002/bjs.1800830107. View

18.

Mauviel A . Cytokine regulation of metalloproteinase gene expression. J Cell Biochem. 1993; 53(4):288-95. DOI: 10.1002/jcb.240530404. View

19.

Murashige M, Miyahara M, Shiraishi N, Saito T, Kohno K, Kobayashi M . Enhanced expression of tissue inhibitors of metalloproteinases in human colorectal tumors. Jpn J Clin Oncol. 1996; 26(5):303-9. DOI: 10.1093/oxfordjournals.jjco.a023237. View

20.

Onisto M, Garbisa S, Caenazzo C, Freda M, Di Francesco C, Nitti D . Reverse transcription-polymerase chain reaction phenotyping of metalloproteinases and inhibitors involved in tumor matrix invasion. Diagn Mol Pathol. 1993; 2(2):74-80. View