Human Transcription Factor HTAF(II)150 (CIF150) is Involved in Transcriptional Regulation of Cell Cycle Progression

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1999 Jul 20

PMID 10409744

Citations 9

Authors

J Martin

R Halenbeck

J Kaufmann

Affiliations

Soon will be listed here.

Abstract

Here we present evidence that CIF150 (hTAF(II)150), the human homolog of Drosophila TAF(II)150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAF(II)150) seems to be less tightly associated with human transcription factor IID than hTAF(II)130 is associated with hTAF(II)250. The transient functional knockout of CIF150 (hTAF(II)150) protein led to cell cycle arrest at the G(2)/M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF(II)150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAF(II)150) directly stimulated cyclin B1 and cyclin A transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAF(II)150) function. We defined a CIF150 (hTAF(II)150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter. These results suggest that one function of CIF150 (hTAF(II)150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.

Citing Articles

RNA polymerase II transcription initiation in holo-TFIID-depleted mouse embryonic stem cells.

Hisler V, Bardot P, Detilleux D, Bernardini A, Stierle M, Sanchez E Cell Rep. 2024; 43(10):114791.

PMID: 39352809 PMC: 11551524. DOI: 10.1016/j.celrep.2024.114791.

TAF2, within the TFIID complex, regulates the expression of a subset of protein-coding genes.

Cheng I, Pi W, Hsu C, Guo Y, Lai J, Wang G Cell Death Discov. 2024; 10(1):244.

PMID: 38773077 PMC: 11109217. DOI: 10.1038/s41420-024-02017-z.

Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription.

Pennington K, Marr S, Chirn G, Marr 2nd M Proc Natl Acad Sci U S A. 2013; 110(19):7678-83.

PMID: 23610421 PMC: 3651496. DOI: 10.1073/pnas.1221712110.

High-resolution comparative genomic hybridization of inflammatory breast cancer and identification of candidate genes.

Bekhouche I, Finetti P, Adelaide J, Ferrari A, Tarpin C, Charafe-Jauffret E PLoS One. 2011; 6(2):e16950.

PMID: 21339811 PMC: 3037286. DOI: 10.1371/journal.pone.0016950.

Switching of the core transcription machinery during myogenesis.

Deato M, Tjian R Genes Dev. 2007; 21(17):2137-49.

PMID: 17704303 PMC: 1950853. DOI: 10.1101/gad.1583407.

References

Dignam J, Lebovitz R, Roeder R . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983; 11(5):1475-89. PMC: 325809. DOI: 10.1093/nar/11.5.1475. View

Oelgeschlager T, Tao Y, Kang Y, Roeder R . Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs. Mol Cell. 1998; 1(6):925-31. DOI: 10.1016/s1097-2765(00)80092-1. View

Oliphant A, Brandl C, Struhl K . Defining the sequence specificity of DNA-binding proteins by selecting binding sites from random-sequence oligonucleotides: analysis of yeast GCN4 protein. Mol Cell Biol. 1989; 9(7):2944-9. PMC: 362762. DOI: 10.1128/mcb.9.7.2944-2949.1989. View

Pines J, Hunter T . Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990; 346(6286):760-3. DOI: 10.1038/346760a0. View

Sekiguchi T, Nohiro Y, Nakamura Y, HISAMOTO N, Nishimoto T . The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol. 1991; 11(6):3317-25. PMC: 360184. DOI: 10.1128/mcb.11.6.3317-3325.1991. View

Lew D, Dulic V, Reed S . Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast. Cell. 1991; 66(6):1197-206. DOI: 10.1016/0092-8674(91)90042-w. View

RAY B, White C, Haber J . The TSM1 gene of Saccharomyces cerevisiae overlaps the MAT locus. Curr Genet. 1991; 20(1-2):25-31. DOI: 10.1007/BF00312761. View

Ruppert S, Wang E, Tjian R . Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature. 1993; 362(6416):175-9. DOI: 10.1038/362175a0. View

Hisatake K, Hasegawa S, Takada R, Nakatani Y, Horikoshi M, Roeder R . The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1. Nature. 1993; 362(6416):179-81. DOI: 10.1038/362179a0. View

10.

Brechot C . Oncogenic activation of cyclin A. Curr Opin Genet Dev. 1993; 3(1):11-8. DOI: 10.1016/s0959-437x(05)80335-1. View

11.

Pines J . Cyclins and cyclin-dependent kinases: take your partners. Trends Biochem Sci. 1993; 18(6):195-7. DOI: 10.1016/0968-0004(93)90185-p. View

12.

Yokomori K, Admon A, Goodrich J, Chen J, Tjian R . Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex. Genes Dev. 1993; 7(11):2235-45. DOI: 10.1101/gad.7.11.2235. View

13.

Verrijzer C, Yokomori K, Chen J, Tjian R . Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA. Science. 1994; 264(5161):933-41. DOI: 10.1126/science.8178153. View

14.

Henglein B, Chenivesse X, Wang J, Eick D, Brechot C . Structure and cell cycle-regulated transcription of the human cyclin A gene. Proc Natl Acad Sci U S A. 1994; 91(12):5490-4. PMC: 44021. DOI: 10.1073/pnas.91.12.5490. View

15.

Martinez E, Chiang C, Ge H, Roeder R . TATA-binding protein-associated factor(s) in TFIID function through the initiator to direct basal transcription from a TATA-less class II promoter. EMBO J. 1994; 13(13):3115-26. PMC: 395202. DOI: 10.1002/j.1460-2075.1994.tb06610.x. View

16.

Kaufmann J, Smale S . Direct recognition of initiator elements by a component of the transcription factor IID complex. Genes Dev. 1994; 8(7):821-9. DOI: 10.1101/gad.8.7.821. View

17.

Purnell B, Emanuel P, Gilmour D . TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes. Genes Dev. 1994; 8(7):830-42. DOI: 10.1101/gad.8.7.830. View

18.

Hunter T, Pines J . Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age. Cell. 1994; 79(4):573-82. DOI: 10.1016/0092-8674(94)90543-6. View

19.

Cogswell J, Godlevski M, Bonham M, Bisi J, Babiss L . Upstream stimulatory factor regulates expression of the cell cycle-dependent cyclin B1 gene promoter. Mol Cell Biol. 1995; 15(5):2782-90. PMC: 230509. DOI: 10.1128/MCB.15.5.2782. View

20.

Johnson A . The price of repression. Cell. 1995; 81(5):655-8. DOI: 10.1016/0092-8674(95)90524-3. View