Cloning of E6 and E7 Genes of Human Papilloma Virus Type 18 and Transformation Potential of E7 Gene and Its Mutants

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 1999 Jul 14

PMID 10403700

Authors

M Laassri

L Gulko

S Vinokurova

N Kisseljova

V Veiko

F Kisseljov

Affiliations

Soon will be listed here.

Abstract

E6 and E7 genes of human papilloma virus type 18 have been subcloned from plasmid pC7, carrying an insert of DNA from squamous cell carcinoma of cervix. Both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. In the case of E6 gene this mutation is mapped in codon 129, in the case of E7 the same change AAC to AAA mapped in codon 92. In addition both genes contain few point mutations that do not change the aminoacid sequences of the protein. Two mutants of E7 gene have been constructed by site directed mutagenesis based on PCR technology-one in codon 10 (change Asp to Asn) and one in codon 24 (change Asp to Gly). The first type of mutation did not influence the transformation potential of the E7 gene in comparison to the parental one with mutation in codon 92. The mutation in codon 24 (region responsible for the interaction with Rb protein) eliminate the transformation potential of the gene. The cells transformed with E7 mutants in codons 10 and 92 were tumorigenic for syngenic rats.

References

Chatis P, Holland C, Silver J, Frederickson T, Hopkins N, Hartley J . A 3' end fragment encompassing the transcriptional enhancers of nondefective Friend virus confers erythroleukemogenicity on Moloney leukemia virus. J Virol. 1984; 52(1):248-54. PMC: 254512. DOI: 10.1128/JVI.52.1.248-254.1984. View

Yoshinaka Y, Katoh I, Copeland T, Oroszlan S . Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon. Proc Natl Acad Sci U S A. 1985; 82(6):1618-22. PMC: 397323. DOI: 10.1073/pnas.82.6.1618. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Holland C, Anklesaria P, Sakakeeny M, Greenberger J . Enhancer sequences of a retroviral vector determine expression of a gene in multipotent hematopoietic progenitors and committed erythroid cells. Proc Natl Acad Sci U S A. 1987; 84(23):8662-6. PMC: 299606. DOI: 10.1073/pnas.84.23.8662. View

DeFranco D, Yamamoto K . Two different factors act separately or together to specify functionally distinct activities at a single transcriptional enhancer. Mol Cell Biol. 1986; 6(4):993-1001. PMC: 367607. DOI: 10.1128/mcb.6.4.993-1001.1986. View

Perryman S, Nishio J, Chesebro B . Complete nucleotide sequence of Friend murine leukemia virus, strain FB29. Nucleic Acids Res. 1991; 19(24):6950. PMC: 329334. DOI: 10.1093/nar/19.24.6950. View

Chatis P, Holland C, Hartley J, Rowe W, Hopkins N . Role for the 3' end of the genome in determining disease specificity of Friend and Moloney murine leukemia viruses. Proc Natl Acad Sci U S A. 1983; 80(14):4408-11. PMC: 384047. DOI: 10.1073/pnas.80.14.4408. View

Sun W, OConnell M, Speck N . Characterization of a protein that binds multiple sequences in mammalian type C retrovirus enhancers. J Virol. 1993; 67(4):1976-86. PMC: 240266. DOI: 10.1128/JVI.67.4.1976-1986.1993. View

Speck N, Baltimore D . Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer. Mol Cell Biol. 1987; 7(3):1101-10. PMC: 365182. DOI: 10.1128/mcb.7.3.1101-1110.1987. View

10.

Ru M, Shustik C, Rassart E . Graffi murine leukemia virus: molecular cloning and characterization of the myeloid leukemia-inducing agent. J Virol. 1993; 67(8):4722-31. PMC: 237858. DOI: 10.1128/JVI.67.8.4722-4731.1993. View

11.

Koch W, Zimmermann W, Oliff A, Friedrich R . Molecular analysis of the envelope gene and long terminal repeat of Friend mink cell focus-inducing virus: implications for the functions of these sequences. J Virol. 1984; 49(3):828-40. PMC: 255544. DOI: 10.1128/JVI.49.3.828-840.1984. View

12.

DesGroseillers L, Jolicoeur P . Mapping the viral sequences conferring leukemogenicity and disease specificity in Moloney and amphotropic murine leukemia viruses. J Virol. 1984; 52(2):448-56. PMC: 254545. DOI: 10.1128/JVI.52.2.448-456.1984. View

13.

Vogt M, Haggblom C, Swift S, Haas M . Envelope gene and long terminal repeat determine the different biological properties of Rauscher, Friend, and Moloney mink cell focus-inducing viruses. J Virol. 1985; 55(1):184-92. PMC: 254914. DOI: 10.1128/JVI.55.1.184-192.1985. View

14.

Herr W . Nucleotide sequence of AKV murine leukemia virus. J Virol. 1984; 49(2):471-8. PMC: 255488. DOI: 10.1128/JVI.49.2.471-478.1984. View

15.

Ishimoto A, Takimoto M, Adachi A, Kakuyama M, Kato S, Kakimi K . Sequences responsible for erythroid and lymphoid leukemia in the long terminal repeats of Friend-mink cell focus-forming and Moloney murine leukemia viruses. J Virol. 1987; 61(6):1861-6. PMC: 254191. DOI: 10.1128/JVI.61.6.1861-1866.1987. View

16.

Delassus S, Sonigo P, Wain-Hobson S . Genetic organization of gibbon ape leukemia virus. Virology. 1989; 173(1):205-13. DOI: 10.1016/0042-6822(89)90236-5. View

17.

Ishimoto A, Adachi A, Sakai K, Matsuyama M . Long terminal repeat of Friend-MCF virus contains the sequence responsible for erythroid leukemia. Virology. 1985; 141(1):30-42. DOI: 10.1016/0042-6822(85)90180-1. View

18.

Shinnick T, Lerner R, Sutcliffe J . Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981; 293(5833):543-8. DOI: 10.1038/293543a0. View

19.

Xiong Y, Eickbush T . Origin and evolution of retroelements based upon their reverse transcriptase sequences. EMBO J. 1990; 9(10):3353-62. PMC: 552073. DOI: 10.1002/j.1460-2075.1990.tb07536.x. View

20.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View