Methanobacterium Thermoautotrophicum RNA Polymerase and Transcription in Vitro

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Jul 10

PMID 10400604

Citations 27

Authors

T J DArcy

W HAUSNER

D E Awery

A M Edwards

M Thomm

J N Reeve

Affiliations

Soon will be listed here.

Abstract

RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum DeltaH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.

Citing Articles

The Targeted Deletion of Genes Responsible for Expression of the Mth60 Fimbriae Leads to Loss of Cell-Cell Connections in Methanothermobacter thermautotrophicus ΔH.

Fink C, Martinez-Cano G, Shuster J, Panzera A, Rennhack K, Rohbohm N Appl Environ Microbiol. 2023; 89(7):e0057523.

PMID: 37310347 PMC: 10370314. DOI: 10.1128/aem.00575-23.

Promoter Motifs in NCLDVs: An Evolutionary Perspective.

Oliveira G, Dos Santos Pereira Andrade A, Rodrigues R, Arantes T, Boratto P, Silva L Viruses. 2017; 9(1).

PMID: 28117683 PMC: 5294985. DOI: 10.3390/v9010016.

The [4Fe-4S] clusters of Rpo3 are key determinants in the post Rpo3/Rpo11 heterodimer formation of RNA polymerase in Methanosarcina acetivorans.

Jennings M, Lessner F, Karr E, Lessner D Microbiologyopen. 2016; 6(1).

PMID: 27557794 PMC: 5300874. DOI: 10.1002/mbo3.399.

Redox-sensitive DNA binding by homodimeric Methanosarcina acetivorans MsvR is modulated by cysteine residues.

Isom C, Turner J, Lessner D, Karr E BMC Microbiol. 2013; 13:163.

PMID: 23865844 PMC: 3729527. DOI: 10.1186/1471-2180-13-163.

The methanogen-specific transcription factor MsvR regulates the fpaA-rlp-rub oxidative stress operon adjacent to msvR in Methanothermobacter thermautotrophicus.

Karr E J Bacteriol. 2010; 192(22):5914-22.

PMID: 20851905 PMC: 2976444. DOI: 10.1128/JB.00816-10.

References

Thomm M, Madon J, Stetter K . DNA-dependent RNA polymerases of the three orders of methanogens. Biol Chem Hoppe Seyler. 1986; 367(6):473-81. DOI: 10.1515/bchm3.1986.367.1.473. View

Holstege F, Young R . Transcriptional regulation: contending with complexity. Proc Natl Acad Sci U S A. 1999; 96(1):2-4. PMC: 33537. DOI: 10.1073/pnas.96.1.2. View

Knaub S, Klein A . Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro. Nucleic Acids Res. 1990; 18(6):1441-6. PMC: 330509. DOI: 10.1093/nar/18.6.1441. View

Edwards A, Kane C, Young R, Kornberg R . Two dissociable subunits of yeast RNA polymerase II stimulate the initiation of transcription at a promoter in vitro. J Biol Chem. 1991; 266(1):71-5. View

Tabassum R, Sandman K, Reeve J . HMt, a histone-related protein from Methanobacterium thermoautotrophicum delta H. J Bacteriol. 1992; 174(24):7890-5. PMC: 207522. DOI: 10.1128/jb.174.24.7890-7895.1992. View

Langer D, Hain J, Thuriaux P, Zillig W . Transcription in archaea: similarity to that in eucarya. Proc Natl Acad Sci U S A. 1995; 92(13):5768-72. PMC: 41582. DOI: 10.1073/pnas.92.13.5768. View

Shpakovski G, Acker J, Wintzerith M, Lacroix J, Thuriaux P, Vigneron M . Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae. Mol Cell Biol. 1995; 15(9):4702-10. PMC: 230713. DOI: 10.1128/MCB.15.9.4702. View

Gohl H, Grondahl B, Thomm M . Promoter recognition in archaea is mediated by transcription factors: identification of transcription factor aTFB from Methanococcus thermolithotrophicus as archaeal TATA-binding protein. Nucleic Acids Res. 1995; 23(19):3837-41. PMC: 307299. DOI: 10.1093/nar/23.19.3837. View

Bult C, White O, Olsen G, Zhou L, Fleischmann R, Sutton G . Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science. 1996; 273(5278):1058-73. DOI: 10.1126/science.273.5278.1058. View

10.

HAUSNER W, Wettach J, Hethke C, Thomm M . Two transcription factors related with the eucaryal transcription factors TATA-binding protein and transcription factor IIB direct promoter recognition by an archaeal RNA polymerase. J Biol Chem. 1996; 271(47):30144-8. DOI: 10.1074/jbc.271.47.30144. View

11.

Nikolov D, Burley S . RNA polymerase II transcription initiation: a structural view. Proc Natl Acad Sci U S A. 1997; 94(1):15-22. PMC: 33652. DOI: 10.1073/pnas.94.1.15. View

12.

Courchesne P, Luethy R, Patterson S . Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption/ionization mass spectrometry. Electrophoresis. 1997; 18(3-4):369-81. DOI: 10.1002/elps.1150180311. View

13.

Qureshi S, Bell S, Jackson S . Factor requirements for transcription in the Archaeon Sulfolobus shibatae. EMBO J. 1997; 16(10):2927-36. PMC: 1169900. DOI: 10.1093/emboj/16.10.2927. View

14.

Sakurai H, Ishihama A . Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II. Gene. 1997; 196(1-2):165-74. DOI: 10.1016/s0378-1119(97)00222-9. View

15.

Reeve J, Nolling J, Morgan R, Smith D . Methanogenesis: genes, genomes, and who's on first?. J Bacteriol. 1997; 179(19):5975-86. PMC: 179496. DOI: 10.1128/jb.179.19.5975-5986.1997. View

16.

Smith D, DeLoughery C, Lee H, Dubois J, Aldredge T, Bashirzadeh R . Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. J Bacteriol. 1997; 179(22):7135-55. PMC: 179657. DOI: 10.1128/jb.179.22.7135-7155.1997. View

17.

Klenk H, Clayton R, Tomb J, White O, Nelson K, Ketchum K . The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature. 1997; 390(6658):364-70. DOI: 10.1038/37052. View

18.

Ouzounis C, Kyrpides N . A ferredoxin-like domain in RNA polymerase 30/40-kDa subunits. Trends Biochem Sci. 1998; 23(5):169-70. DOI: 10.1016/s0968-0004(98)01203-1. View

19.

Kawarabayasi Y, Sawada M, Horikawa H, Haikawa Y, Hino Y, Yamamoto S . Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3 (supplement). DNA Res. 1998; 5(2):147-55. DOI: 10.1093/dnares/5.2.147. View

20.

Myer V, Young R . RNA polymerase II holoenzymes and subcomplexes. J Biol Chem. 1998; 273(43):27757-60. DOI: 10.1074/jbc.273.43.27757. View