Significance of Fatty Acids in Pregnancy-induced Immunosuppression

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1999 Jul 3

PMID 10391868

Citations 8

Authors

I Crocker

N Lawson

I Daniels

P Baker

J Fletcher

Affiliations

Soon will be listed here.

Abstract

Pregnancy can exert suppressive effects on chronic inflammatory conditions. We have previously demonstrated a depression in polymorphonuclear leukocyte (PMN) respiratory burst during pregnancy which could explain this amelioration. To elucidate the biochemical mechanism, we have examined PMN phospholipase A2 (PLA2) activity and its relationship to cellular and circulating fatty acids in pregnant women (30 to 34 weeks) and nonpregnant controls. PMN PLA2 activity was determined by arachidonic acid (AA) and leukotriene B4 (LTB4) release, respiratory burst activity was determined by lucigenin-enhanced chemiluminescence, and total serum and PMN fatty acid levels were determined by gas-liquid chromatography. AA release was significantly reduced for pregnancy PMNs in response to N-formyl-met-leu-phe (fMLP) under unprimed and tumor necrosis factor alpha (TNF-alpha)- or interleukin 8-primed conditions. Similarly, LTB4 liberation was significantly reduced in response to fMLP and phorbol myristate acetate in unprimed and TNF-alpha-primed pregnancy PMNs. All major fatty acid classes were altered in the pregnant state. Of these differences in PMNs, oleic acid and alpha-linolenic acid showed a significant increase (13 and 26%, respectively) and stearic acid and AA showed a significant decrease (8 and 30%, respectively). The stearic acid, oleic acid, and AA compositions of all cells analyzed correlated with their corresponding changes in serum fatty acid levels. Crossover serum incubations modified both fatty acid profiles and the PMN respiratory burst accordingly, while individual fatty acid incorporation studies highlighted the importance of polyunsaturated fatty acids for NADPH oxidase efficiency. These findings indicate that the attenuation of PMN function in pregnancy may originate from a reduction in the available pool of cellular fatty acids. Furthermore, this reduction arises as a direct result of a pregnancy-induced shift in circulating fatty acids from polyunsaturated to monounsaturated forms.

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References

DEWALD B, Baggiolini M . Activation of NADPH oxidase in human neutrophils. Synergism between fMLP and the neutrophil products PAF and LTB4. Biochem Biophys Res Commun. 1985; 128(1):297-304. DOI: 10.1016/0006-291x(85)91678-x. View

Sanjurjo P, Matorras R, Ingunza N, Alonso M, Perteagudo L . Cross-sectional study of percentual changes in total plasmatic fatty acids during pregnancy. Horm Metab Res. 1993; 25(11):590-2. DOI: 10.1055/s-2007-1002183. View

Mahoney E, Hamill A, Scott W, COHN Z . Response of endocytosis to altered fatty acyl composition of macrophage phospholipids. Proc Natl Acad Sci U S A. 1977; 74(11):4895-9. PMC: 432063. DOI: 10.1073/pnas.74.11.4895. View

Boyum A . Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968; 97:77-89. View

Beilin L, Croft K, Michael C, Ritchie J, Schmidt L, VANDONGEN R . Neutrophil platelet-activating factor in normal and hypertensive pregnancy and in pregnancy-induced hypertension. Clin Sci (Lond). 1993; 85(1):63-70. DOI: 10.1042/cs0850063. View

Cockcroft S, Stutchfield J . The receptors for ATP and fMetLeuPhe are independently coupled to phospholipases C and A2 via G-protein(s). Relationship between phospholipase C and A2 activation and exocytosis in HL60 cells and human neutrophils. Biochem J. 1989; 263(3):715-23. PMC: 1133491. DOI: 10.1042/bj2630715. View

Spector T, Da Silva J . Pregnancy and rheumatoid arthritis: an overview. Am J Reprod Immunol. 1992; 28(3-4):222-5. DOI: 10.1111/j.1600-0897.1992.tb00797.x. View

Finn R, St Hill C, Govan A, Ralfs I, Gurney F, Denye V . Immunological responses in pregnancy and survival of fetal homograft. Br Med J. 1972; 3(5819):150-2. PMC: 1788602. DOI: 10.1136/bmj.3.5819.150. View

Persellin R, Leibfarth J . Studies of the effects of pregnancy serum on polymorphonuclear leukocyte functions. Arthritis Rheum. 1978; 21(3):316-25. DOI: 10.1002/art.1780210305. View

10.

Flesch I, FERBER E . Effect of cellular fatty acid composition on the phospholipase A2 activity of bone marrow-derived macrophages, and their ability to induce lucigenin-dependent chemiluminescence. Biochim Biophys Acta. 1986; 889(1):6-14. DOI: 10.1016/0167-4889(86)90003-0. View

11.

Bjorksten B, Soderstrom T, Damber M, von Schoultz B, Stigbrand T . Polymorphonuclear leucocyte function during pregnancy. Scand J Immunol. 1978; 8(3):257-62. DOI: 10.1111/j.1365-3083.1978.tb00518.x. View

12.

Brabin B . Epidemiology of infection in pregnancy. Rev Infect Dis. 1985; 7(5):579-603. DOI: 10.1093/clinids/7.5.579. View

13.

Luft B, Remington J . Effect of pregnancy on resistance to Listeria monocytogenes and Toxoplasma gondii infections in mice. Infect Immun. 1982; 38(3):1164-71. PMC: 347871. DOI: 10.1128/iai.38.3.1164-1171.1982. View

14.

Barnette M, Rush J, Marshall L, FOLEY J, Schmidt D, Sarau H . Effects of scalaradial, a novel inhibitor of 14 kDa phospholipase A2, on human neutrophil function. Biochem Pharmacol. 1994; 47(9):1661-7. DOI: 10.1016/0006-2952(94)90545-2. View

15.

Lepage G, Roy C . Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res. 1986; 27(1):114-20. View

16.

ANDRESEN R, MONROE C . Experimental study of the behavior of adult human skin homografts during pregnancy. A preliminary report. Am J Obstet Gynecol. 1962; 84:1096-103. DOI: 10.1016/0002-9378(62)90560-4. View

17.

Persellin R . The effect of pregnancy on rheumatoid arthritis. Bull Rheum Dis. 1976; 27(9):922-7. View

18.

Hempel K, Fernandez L, Persellin R . Effect of pregnancy sera on isolated lysosomes. Nature. 1970; 225(5236):955-6. DOI: 10.1038/225955a0. View

19.

Dana R, Malech H, Levy R . The requirement for phospholipase A2 for activation of the assembled NADPH oxidase in human neutrophils. Biochem J. 1994; 297 ( Pt 1):217-23. PMC: 1137813. DOI: 10.1042/bj2970217. View

20.

Fletcher J . Impaired neutrophil function and myeloperoxidase deficiency in pregnancy. Br J Haematol. 1980; 44(3):375-81. DOI: 10.1111/j.1365-2141.1980.tb05906.x. View