Microsatellite Instability and Mismatch Repair Gene Inactivation in Sporadic Pancreatic and Colon Tumours

Overview

Journal Br J Cancer

Specialty Oncology

Date 1999 Jul 2

PMID 10389971

Citations 17

Authors

C Ghimenti

P Tannergard

S Wahlberg

T Liu

P G Giulianotti

F Mosca

G Fornaciari

G Bevilacqua

A Lindblom

M A Caligo

Affiliations

Soon will be listed here.

Abstract

Genomic instability has been proposed as a new mechanism of carcinogenesis involved in hereditary non-polyposis colorectal cancer (HNPCC) and in a large number of sporadic cancers like pancreatic and colon tumours. Mutations in human mismatch repair genes have been found in HNPCC patients, but their involvement in sporadic cancer has not been clarified yet. In this study we screened 21 pancreatic and 23 colorectal sporadic cancers for microsatellite instability by ten and six different microsatellite markers respectively. Microsatellite alterations were observed at one or more loci in 66.6% (14/21) of pancreatic cancers and in 26% (6/23) colon tumours, but all the pancreatic and half of the colon samples showed a low rate of microsatellite instability. All the unstable samples were further analysed for mutations in the hMLH1 and hMSH2 genes and for hypermethylation of the hMLH1 promoter region. Alterations in the hMLH1 gene were found only in colorectal tumours with a large presence of microsatellite instability. None of the pancreatic tumours showed any alteration in the two genes analysed. Our results demonstrate that microsatellite instability is unlikely to play a role in the tumorigenesis of sporadic pancreatic cancers and confirm the presence of mismatch repair gene alterations only in sporadic colon tumours with a highly unstable phenotype.

Citing Articles

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Immune-Based Therapies and the Role of Microsatellite Instability in Pancreatic Cancer.

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MMR Deficiency is Homogeneous in Pancreatic Carcinoma and Associated with High Density of Cd8-Positive Lymphocytes.

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Kim S, Shin Y, Kim S, Seo H, Kwon W, Kim H Pancreas. 2019; 48(10):1285-1293.

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