B Lymphocyte Galactosyltransferase Protein Levels in Normal Individuals and in Patients with Rheumatoid Arthritis

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 1999 Jul 1

PMID 10386894

Citations 9

Authors

J Keusch

P M Lydyard

E G Berger

P J Delves

Affiliations

Soon will be listed here.

Abstract

We have quantified the level of beta4-galactosyltransferase protein in human B lymphocytes using an ELISA-based assay. Between 1-10ng of beta4-galactosyltransferase was detected per mg total cellular protein, indicating that this enzyme constitutes <0.001% of B lymphocyte cellular protein. Akin to previous studies, individuals with rheumatoid arthritis exhibited reduced lymphocytic galactosyltransferase enzyme activity compared with normal controls when using ovalbumin as the acceptor substrate. The levels of enzyme protein present in B lymphocytes from patients with rheumatoid arthritis was, however, not reduced suggesting that the B lymphocyte galactosyltransferase catalytic activity may be regulated post-translationally.

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References

Sumar N, Bodman K, Rademacher T, Dwek R, Williams P, Parekh R . Analysis of glycosylation changes in IgG using lectins. J Immunol Methods. 1990; 131(1):127-36. DOI: 10.1016/0022-1759(90)90242-n. View

Varki A . Biological roles of oligosaccharides: all of the theories are correct. Glycobiology. 1993; 3(2):97-130. PMC: 7108619. DOI: 10.1093/glycob/3.2.97. View

Jeddi P, Bodman-Smith K, Lund T, Lydyard P, Isenberg D, Youinou P . Agalactosyl IgG and beta-1,4-galactosyltransferase gene expression in rheumatoid arthritis patients and in the arthritis-prone MRL lpr/lpr mouse. Immunology. 1996; 87(4):654-9. PMC: 1384147. DOI: 10.1046/j.1365-2567.1996.474593.x. View

Wilson I, Platt F, Isenberg D, Rademacher T . Aberrant control of galactosyltransferase in peripheral B lymphocytes and Epstein-Barr virus transformed B lymphoblasts from patients with rheumatoid arthritis. J Rheumatol. 1993; 20(8):1282-7. View

Watzele G, Bachofner R, Berger E . Immunocytochemical localization of the Golgi apparatus using protein-specific antibodies to galactosyltransferase. Eur J Cell Biol. 1991; 56(2):451-8. View

Keusch J, Lydyard P, Isenberg D, Delves P . beta 1,4-Galactosyltransferase activity in B cells detected using a simple ELISA-based assay. Glycobiology. 1995; 5(4):365-700. DOI: 10.1093/glycob/5.4.365. View

Rademacher T, Williams P, Dwek R . Agalactosyl glycoforms of IgG autoantibodies are pathogenic. Proc Natl Acad Sci U S A. 1994; 91(13):6123-7. PMC: 44150. DOI: 10.1073/pnas.91.13.6123. View

Lo N, Shaper J, Pevsner J, Shaper N . The expanding beta 4-galactosyltransferase gene family: messages from the databanks. Glycobiology. 1998; 8(5):517-26. DOI: 10.1093/glycob/8.5.517. View

Wright A, Morrison S . Effect of C2-associated carbohydrate structure on Ig effector function: studies with chimeric mouse-human IgG1 antibodies in glycosylation mutants of Chinese hamster ovary cells. J Immunol. 1998; 160(7):3393-402. View

10.

van Zeben D, Rook G, Hazes J, Zwinderman A, Zhang Y, Ghelani S . Early agalactosylation of IgG is associated with a more progressive disease course in patients with rheumatoid arthritis: results of a follow-up study. Br J Rheumatol. 1994; 33(1):36-43. DOI: 10.1093/rheumatology/33.1.36. View

11.

Parekh R, Isenberg D, Rook G, Roitt I, Dwek R, Rademacher T . A comparative analysis of disease-associated changes in the galactosylation of serum IgG. J Autoimmun. 1989; 2(2):101-14. DOI: 10.1016/0896-8411(89)90148-0. View

12.

Gerber A, Kozdrowski I, Wyss S, Berger E . The charge heterogeneity of soluble human galactosyltransferases isolated from milk, amniotic fluid and malignant ascites. Eur J Biochem. 1979; 93(3):453-60. DOI: 10.1111/j.1432-1033.1979.tb12843.x. View

13.

Axford J, Mackenzie L, Lydyard P, Hay F, Isenberg D, Roitt I . Reduced B-cell galactosyltransferase activity in rheumatoid arthritis. Lancet. 1987; 2(8574):1486-8. DOI: 10.1016/s0140-6736(87)92621-3. View

14.

Strous G, van Kerkhof P, Fallon R, Schwartz A . Golgi galactosyltransferase contains serine-linked phosphate. Eur J Biochem. 1987; 169(2):307-11. DOI: 10.1111/j.1432-1033.1987.tb13613.x. View

15.

Sato T, Furukawa K, Bakker H, van den Eijnden D, van Die I . Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase. Proc Natl Acad Sci U S A. 1998; 95(2):472-7. PMC: 18444. DOI: 10.1073/pnas.95.2.472. View

16.

Furukawa K, Matsuta K, Takeuchi F, Kosuge E, Miyamoto T, Kobata A . Kinetic study of a galactosyltransferase in the B cells of patients with rheumatoid arthritis. Int Immunol. 1990; 2(1):105-12. DOI: 10.1093/intimm/2.1.105. View

17.

Arnett F, Edworthy S, Bloch D, McShane D, Fries J, Cooper N . The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988; 31(3):315-24. DOI: 10.1002/art.1780310302. View

18.

Bond A, Alavi A, Axford J, Bourke B, Bruckner F, Kerr M . A detailed lectin analysis of IgG glycosylation, demonstrating disease specific changes in terminal galactose and N-acetylglucosamine. J Autoimmun. 1997; 10(1):77-85. DOI: 10.1006/jaut.1996.0104. View

19.

Winfield J, Jarjour W . Stress proteins in autoimmunity. Adv Exp Med Biol. 1994; 347:99-113. DOI: 10.1007/978-1-4615-2427-4_11. View

20.

Berger E, Grimm K, Bachi T, Bosshart H, Kleene R, Watzele M . Double immunofluorescent staining of alpha 2,6 sialyltransferase and beta 1,4 galactosyltransferase in monensin-treated cells: evidence for different Golgi compartments?. J Cell Biochem. 1993; 52(3):275-88. DOI: 10.1002/jcb.240520304. View