Molecular Cloning and Characterization of a Locus Responsible for O Acetylation of the O Polysaccharide of Legionella Pneumophila Serogroup 1 Lipopolysaccharide
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Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide.
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