Immunological Method to Differentiate Between Antigens of Tubercle Bacilli, Other Mycobacterial Species, and Non-acid-fast Bacteria

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1978 Oct 1

PMID 103823

Citations 4

Authors

H R Ferguson

J K McClatchy

T R Sharpton

P MINDEN

Affiliations

Soon will be listed here.

Abstract

Sera from rabbits immunized with sonicates of Mycobacterium bovis BCG were passed through an immunoadsorbent made of a soluble BCG extract to make partially purified antibodies to BCG. These antibodies were in turn used to prepare an immunoadsorbent through which the BCG extract was passed. The partially purified antigenic material was radiolabeled and subjected to electrophoresis in acrylamide gels. One of the radiolabeled fractions isolated (BCG-C) was found to bind to antibodies to BCG and H37Rv, but not to antibodies in sera from rabbits immunized with other mycobacterial species or Nocardia asteroides. The reaction between BCG-C and the partially purified antibodies to BCG was inhibited by small amounts of different BCG antigens. Cultures obtained from 25 patients with tuberculous diseases, other bacterial cultures, and various bacterial extracts were tested for their capacity to inhibit this reaction. Each of 13 mycobacteria identified as M. tuberculosis inhibited this reaction. Equivalent numbers of 12 strains of mycobacteria other than M. tuberculosis and high concentrations of other bacterial extracts did not inhibit, indicating that determinants of BCG present in M. tuberculosis were not detected in the other mycobacteria or in non-acid-fast bacteria. The use of sequential purification procedures could be of potential clinical value in quickly differentiating between M. tuberculosis and a variety of other mycobacteria.

Citing Articles

Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG.

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Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG.

MINDEN P, Kelleher P, Freed J, Nielsen L, Brennan P, McPheron L Infect Immun. 1984; 46(2):519-25.

PMID: 6389346 PMC: 261565. DOI: 10.1128/iai.46.2.519-525.1984.

Clinical and clinical laboratory aspects of nocardial infection.

Stevens D J Hyg (Lond). 1983; 91(3):377-84.

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Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis.

Wayne L, Sramek H Infect Immun. 1979; 24(2):363-70.

PMID: 110697 PMC: 414310. DOI: 10.1128/iai.24.2.363-370.1979.

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