A Uve1p-mediated Mismatch Repair Pathway in Schizosaccharomyces Pombe

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1999 Jun 22

PMID 10373519

Citations 13

Authors

B Kaur

J L Fraser

G A Freyer

S Davey

P W Doetsch

Affiliations

Soon will be listed here.

Abstract

UV damage endonuclease (Uve1p) from Schizosaccharomyces pombe was initially described as a DNA repair enzyme specific for the repair of UV light-induced photoproducts and proposed as the initial step in an alternative excision repair pathway. Here we present biochemical and genetic evidence demonstrating that Uve1p is also a mismatch repair endonuclease which recognizes and cleaves DNA 5' to the mispaired base in a strand-specific manner. The biochemical properties of the Uve1p-mediated mismatch endonuclease activity are similar to those of the Uve1p-mediated UV photoproduct endonuclease. Mutants lacking Uve1p display a spontaneous mutator phenotype, further confirming the notion that Uve1p plays a role in mismatch repair. These results suggest that Uve1p has a surprisingly broad substrate specificity and may function as a general type of DNA repair protein with the capacity to initiate mismatch repair in certain organisms.

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