RYBP, a New Repressor Protein That Interacts with Components of the Mammalian Polycomb Complex, and with the Transcription Factor YY1

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1999 Jun 16

PMID 10369680

Citations 98

Authors

E Garcia

M del Mar Lorente

J C Moreno

M Vidal

Affiliations

Soon will be listed here.

Abstract

The products of the Polycomb group (PcG) of genes are necessary for the maintenance of transcriptional repression of a number of important developmental genes, including the homeotic genes. A two-hybrid screen was used to search for putative new members of the PcG of genes in mammals. We have identified a new Zn finger protein, RYBP, which interacts directly with both Ring1 proteins (Ring1A and Ring1B) and with M33, two mutually interacting sets of proteins of the mammalian Polycomb complex. Ring1 binds RYBP and M33 through the same C-terminal domain, whereas the RYBP-M33 interaction takes place through an M33 domain not involved in Ring1 binding. RYBP also interacts directly with YY1, a transcription factor partially related to the product of the Drosophila pleiohomeotic gene. In addition, we show here that RYBP acts as a transcriptional repressor in transiently transfected cells. Finally, RYBP shows a dynamic expression pattern during embryogenesis which initially overlaps partially that of Ring1A in the central nervous system, and later becomes ubiquitous. Taken together, these data suggest that RYBP may play a relevant role in PcG function in mammals.

Citing Articles

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Unveiling the Binding between the Armadillo-Repeat Domain of Plakophilin 1 and the Intrinsically Disordered Transcriptional Repressor RYBP.

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Structural basis of the histone ubiquitination read-write mechanism of RYBP-PRC1.

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References

Alkema M, van der Lugt N, Bobeldijk R, Berns A, van Lohuizen M . Transformation of axial skeleton due to overexpression of bmi-1 in transgenic mice. Nature. 1995; 374(6524):724-7. DOI: 10.1038/374724a0. View

Hollenberg S, Sternglanz R, Cheng P, Weintraub H . Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system. Mol Cell Biol. 1995; 15(7):3813-22. PMC: 230620. DOI: 10.1128/MCB.15.7.3813. View

Spanopoulou E, Zaitseva F, Wang F, Santagata S, Baltimore D, Panayotou G . The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination. Cell. 1996; 87(2):263-76. DOI: 10.1016/s0092-8674(00)81344-6. View

Alkema M, Bronk M, Verhoeven E, Otte A, van t Veer L, Berns A . Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex. Genes Dev. 1997; 11(2):226-40. DOI: 10.1101/gad.11.2.226. View

Hobert O, Jallal B, Ullrich A . Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression. Mol Cell Biol. 1996; 16(6):3066-73. PMC: 231301. DOI: 10.1128/MCB.16.6.3066. View

Bienz M, Muller J . Transcriptional silencing of homeotic genes in Drosophila. Bioessays. 1995; 17(9):775-84. DOI: 10.1002/bies.950170907. View

Coulson M, Robert S, Eyre H, Saint R . The identification and localization of a human gene with sequence similarity to Polycomblike of Drosophila melanogaster. Genomics. 1998; 48(3):381-3. DOI: 10.1006/geno.1997.5201. View

Grossniklaus U, Hoeppner M, Gagliano W . Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis. Science. 1998; 280(5362):446-50. DOI: 10.1126/science.280.5362.446. View

Buchenau P, Hodgson J, Strutt H, Arndt-Jovin D . The distribution of polycomb-group proteins during cell division and development in Drosophila embryos: impact on models for silencing. J Cell Biol. 1998; 141(2):469-81. PMC: 2148446. DOI: 10.1083/jcb.141.2.469. View

10.

Kyba M, Brock H . The Drosophila polycomb group protein Psc contacts ph and Pc through specific conserved domains. Mol Cell Biol. 1998; 18(5):2712-20. PMC: 110650. DOI: 10.1128/MCB.18.5.2712. View

11.

van de Vosse E, Walpole S, Nicolaou A, van der Bent P, Cahn A, Vaudin M . Characterization of SCML1, a new gene in Xp22, with homology to developmental polycomb genes. Genomics. 1998; 49(1):96-102. DOI: 10.1006/geno.1998.5224. View

12.

Hashimoto N, Brock H, Nomura M, Kyba M, Hodgson J, Fujita Y . RAE28, BMI1, and M33 are members of heterogeneous multimeric mammalian Polycomb group complexes. Biochem Biophys Res Commun. 1998; 245(2):356-65. DOI: 10.1006/bbrc.1998.8438. View

13.

Pirrotta V . Polycombing the genome: PcG, trxG, and chromatin silencing. Cell. 1998; 93(3):333-6. DOI: 10.1016/s0092-8674(00)81162-9. View

14.

van Lohuizen M, Tijms M, Voncken J, Schumacher A, Magnuson T, Wientjens E . Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes. Mol Cell Biol. 1998; 18(6):3572-9. PMC: 108938. DOI: 10.1128/MCB.18.6.3572. View

15.

Sewalt R, van der Vlag J, Gunster M, Hamer K, den Blaauwen J, Satijn D . Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes. Mol Cell Biol. 1998; 18(6):3586-95. PMC: 108940. DOI: 10.1128/MCB.18.6.3586. View

16.

Holdeman R, Nehrt S, Strome S . MES-2, a maternal protein essential for viability of the germline in Caenorhabditis elegans, is homologous to a Drosophila Polycomb group protein. Development. 1998; 125(13):2457-67. DOI: 10.1242/dev.125.13.2457. View

17.

Korf I, Fan Y, Strome S . The Polycomb group in Caenorhabditis elegans and maternal control of germline development. Development. 1998; 125(13):2469-78. DOI: 10.1242/dev.125.13.2469. View

18.

Hemenway C, Halligan B, Levy L . The Bmi-1 oncoprotein interacts with dinG and MPh2: the role of RING finger domains. Oncogene. 1998; 16(19):2541-7. DOI: 10.1038/sj.onc.1202042. View

19.

Brown J, Mucci D, Whiteley M, Dirksen M, Kassis J . The Drosophila Polycomb group gene pleiohomeotic encodes a DNA binding protein with homology to the transcription factor YY1. Mol Cell. 1998; 1(7):1057-64. DOI: 10.1016/s1097-2765(00)80106-9. View

20.

Mihaly J, Mishra R, Karch F . A conserved sequence motif in Polycomb-response elements. Mol Cell. 1998; 1(7):1065-6. DOI: 10.1016/s1097-2765(00)80107-0. View