Functioning of DcuC As the C4-dicarboxylate Carrier During Glucose Fermentation by Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Jun 15

PMID 10368146

Citations 15

Authors

E Zientz

I G Janausch

S Six

G Unden

Affiliations

Soon will be listed here.

Abstract

The dcuC gene of Escherichia coli encodes an alternative C4-dicarboxylate carrier (DcuC) with low transport activity. The expression of dcuC was investigated. dcuC was expressed only under anaerobic conditions; nitrate and fumarate caused slight repression and stimulation of expression, respectively. Anaerobic induction depended mainly on the transcriptional regulator FNR. Fumarate stimulation was independent of the fumarate response regulator DcuR. The expression of dcuC was not significantly inhibited by glucose, assigning a role to DcuC during glucose fermentation. The inactivation of dcuC increased fumarate-succinate exchange and fumarate uptake by DcuA and DcuB, suggesting a preferential function of DcuC in succinate efflux during glucose fermentation. Upon overexpression in a dcuC promoter mutant (dcuC*), DcuC was able to compensate for DcuA and DcuB in fumarate-succinate exchange and fumarate uptake.

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References

Stewart V . Nitrate regulation of anaerobic respiratory gene expression in Escherichia coli. Mol Microbiol. 1993; 9(3):425-34. DOI: 10.1111/j.1365-2958.1993.tb01704.x. View

Six S, Andrews S, Unden G, Guest J . Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct). J Bacteriol. 1994; 176(21):6470-8. PMC: 197000. DOI: 10.1128/jb.176.21.6470-6478.1994. View

Lu Z, Cabiscol E, Obradors N, Tamarit J, Ros J, Aguilar J . Evolution of an Escherichia coli protein with increased resistance to oxidative stress. J Biol Chem. 1998; 273(14):8308-16. DOI: 10.1074/jbc.273.14.8308. View

Ostrow K, Silhavy T, Garrett S . cis-acting sites required for osmoregulation of ompF expression in Escherichia coli K-12. J Bacteriol. 1986; 168(3):1165-71. PMC: 213618. DOI: 10.1128/jb.168.3.1165-1171.1986. View

Li J, Kustu S, Stewart V . In vitro interaction of nitrate-responsive regulatory protein NarL with DNA target sequences in the fdnG, narG, narK and frdA operon control regions of Escherichia coli K-12. J Mol Biol. 1994; 241(2):150-65. DOI: 10.1006/jmbi.1994.1485. View

Jayaraman P, Cole J, Busby S . Mutational analysis of the nucleotide sequence at the FNR-dependent nirB promoter in Escherichia coli. Nucleic Acids Res. 1989; 17(1):135-45. PMC: 331540. DOI: 10.1093/nar/17.1.135. View

Iuchi S, Lin E . Adaptation of Escherichia coli to redox environments by gene expression. Mol Microbiol. 1993; 9(1):9-15. DOI: 10.1111/j.1365-2958.1993.tb01664.x. View

Engel P, Kramer R, Unden G . Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from the aerobic dicarboxylate uptake system. J Bacteriol. 1992; 174(17):5533-9. PMC: 206496. DOI: 10.1128/jb.174.17.5533-5539.1992. View

Saier Jr M . Molecular phylogeny as a basis for the classification of transport proteins from bacteria, archaea and eukarya. Adv Microb Physiol. 1999; 40:81-136. DOI: 10.1016/s0065-2911(08)60130-7. View

10.

Golby P, Kelly D, Guest J, Andrews S . Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli. J Bacteriol. 1998; 180(24):6586-96. PMC: 107762. DOI: 10.1128/JB.180.24.6586-6596.1998. View

11.

Bongaerts J, Zoske S, Weidner U, Unden G . Transcriptional regulation of the proton translocating NADH dehydrogenase genes (nuoA-N) of Escherichia coli by electron acceptors, electron donors and gene regulators. Mol Microbiol. 1995; 16(3):521-34. DOI: 10.1111/j.1365-2958.1995.tb02416.x. View

12.

Zientz E, Six S, Unden G . Identification of a third secondary carrier (DcuC) for anaerobic C4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange. J Bacteriol. 1996; 178(24):7241-7. PMC: 178639. DOI: 10.1128/jb.178.24.7241-7247.1996. View

13.

Engel P, Kramer R, Unden G . Transport of C4-dicarboxylates by anaerobically grown Escherichia coli. Energetics and mechanism of exchange, uptake and efflux. Eur J Biochem. 1994; 222(2):605-14. DOI: 10.1111/j.1432-1033.1994.tb18903.x. View

14.

Zientz E, Bongaerts J, Unden G . Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system. J Bacteriol. 1998; 180(20):5421-5. PMC: 107591. DOI: 10.1128/JB.180.20.5421-5425.1998. View

15.

Sawers G, Suppmann B . Anaerobic induction of pyruvate formate-lyase gene expression is mediated by the ArcA and FNR proteins. J Bacteriol. 1992; 174(11):3474-8. PMC: 206030. DOI: 10.1128/jb.174.11.3474-3478.1992. View

16.

Lynch A, Lin E . Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters. J Bacteriol. 1996; 178(21):6238-49. PMC: 178496. DOI: 10.1128/jb.178.21.6238-6249.1996. View

17.

Davies S, Golby P, Omrani D, Broad S, Harrington V, Guest J . Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli. J Bacteriol. 1999; 181(18):5624-35. PMC: 94081. DOI: 10.1128/JB.181.18.5624-5635.1999. View

18.

Schnetz K, Rak B . IS5: a mobile enhancer of transcription in Escherichia coli. Proc Natl Acad Sci U S A. 1992; 89(4):1244-8. PMC: 48425. DOI: 10.1073/pnas.89.4.1244. View

19.

Bowen S, Hassan H . Characterization of cis-acting regulatory mutations causing anaerobic expression of the sodA gene in Escherichia coli. Arch Biochem Biophys. 1993; 302(2):372-9. DOI: 10.1006/abbi.1993.1226. View

20.

Tran Q, Bongaerts J, Vlad D, Unden G . Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications. Eur J Biochem. 1997; 244(1):155-60. DOI: 10.1111/j.1432-1033.1997.00155.x. View