Chrysotile-induced Asbestosis: Changes in the Free Cell Population, Pulmonary Surfactant and Whole Lung Tissue of Rats

Overview

Journal Br J Exp Pathol

Specialty Pathology

Date 1976 Oct 1

PMID 1036714

Citations 18

Authors

T D Tetley

P M Hext

R J Richards

M McDermott

Affiliations

Soon will be listed here.

Abstract

Rats inhaling chrysotile asbestos contracted asbestosis and fibrosis of the lungs. Studies of biochemical and morphological changes (between normal and treated animals) show that chrysotile induces an increase in the lung free cell population and pulmonary surfactant levels. Lysosomal enzyme levels are elevated in both the whole lung and free cell population and there are considerable changes in macrophage morphology. It is suggested that the primary response of the lung to chrysotile is an increase in surfactant production coupled with an increase in free cell numbers, in order to prevent the cytotoxic effect of the dust.

Citing Articles

Biomarkers of genotoxic damage in pulmonary alveolar macrophages: a review.

DAgostini F, La Maestra S Arch Toxicol. 2024; 99(1):211-224.

PMID: 39453481 DOI: 10.1007/s00204-024-03894-0.

Asbestos-induced lung disease.

Brody A Environ Health Perspect. 1993; 100:21-30.

PMID: 8354168 PMC: 1519571. DOI: 10.1289/ehp.9310021.

Interstitial accumulation of inhaled chrysotile asbestos fibers and consequent formation of microcalcifications.

Brody A, Hill L Am J Pathol. 1982; 109(1):107-14.

PMID: 7124904 PMC: 1916069.

Biochemical and cellular reaction of PVS paste polymers and latex following intratracheal instillation into rats.

Tetley T, ROSE F, Richards R Inflammation. 1981; 5(2):137-52.

PMID: 7024126 DOI: 10.1007/BF00914203.

Interactions of chrysotile asbestos fibres with the complement system.

Saint-Remy J, Cole P Immunology. 1980; 41(2):431-7.

PMID: 6904360 PMC: 1458176.

References

Desai R, Hext P, Richards R . The prevention of asbestos-induced hemolysis. Life Sci. 1975; 16(12):1931-8. DOI: 10.1016/0024-3205(75)90303-3. View

Harwood J, Desai R, Hext P, Tetley T, Richards R . Characterization of pulmonary surfactant from ox, rabbit, rat and sheep. Biochem J. 1975; 151(3):707-14. PMC: 1172420. DOI: 10.1042/bj1510707. View

HEPPLESTON A, Fletcher K, Wyatt I . Changes in the composition of lung lipids and the "turnover" of dipalmitoyl lecithin in experimental alveolar lipo-proteinosis induced by inhaled quartz. Br J Exp Pathol. 1974; 55(4):384-95. PMC: 2072654. View

Allison A, Harington J, Birbeck M . An examination of the cytotoxic effects of silica on macrophages. J Exp Med. 1966; 124(2):141-54. PMC: 2180474. DOI: 10.1084/jem.124.2.141. View

HEPPLESTON A, Styles J . Activity of a macrophage factor in collagen formation by silica. Nature. 1967; 214(5087):521-2. DOI: 10.1038/214521a0. View

MACNAB G, Harington J . Haemolytic activity of asbestos and other mineral dusts. Nature. 1967; 214(5087):522-3. DOI: 10.1038/214522a0. View

Davis J . The fibrogenic effects of mineral dusts injected into the pleural cavity of mice. Br J Exp Pathol. 1972; 53(2):190-201. PMC: 2072548. View

Harington J, Ritchie M, King P, Miller K . The in-vitro effects of silica-treated hamster macrophages on collagen production by hamster fibroblasts. J Pathol. 1973; 109(1):21-37. DOI: 10.1002/path.1711090104. View

Wagner J, Berry G, Timbrell V . Mesotheliomata in rats after inoculation with asbestos and other materials. Br J Cancer. 1973; 28(2):173-85. PMC: 2008881. DOI: 10.1038/bjc.1973.134. View

10.

Burrell R, Anderson M . The induction of fibrogenesis by silica-treated alveolar macrophages. Environ Res. 1973; 6(4):389-94. DOI: 10.1016/0013-9351(73)90054-6. View

11.

Richards R, Wusteman F . The effects of silica dust and alveolar macrophages on lung fibroblasts grown in vitro. Life Sci. 1974; 14(2):355-64. DOI: 10.1016/0024-3205(74)90066-6. View

12.

Wagner J, Berry G, Skidmore J, Timbrell V . The effects of the inhalation of asbestos in rats. Br J Cancer. 1974; 29(3):252-69. PMC: 2009089. DOI: 10.1038/bjc.1974.65. View

13.

Davies P, Allison A, Ackerman J, Butterfield A, Williams S . Asbestos induces selective release of lysosomal enzymes from mononuclear phagocytes. Nature. 1974; 251(5474):423-5. DOI: 10.1038/251423a0. View

14.

Richards R, Hext P, Blundell G, Henderson W, Volcani B . Ultrastructural changes in lung fibroblast cultures exposed to chrysotile asbestos. Br J Exp Pathol. 1974; 55(3):275-81. PMC: 2072625. View

15.

Richards G . Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem. 1974; 57(2):369-76. DOI: 10.1016/0003-2697(74)90091-8. View

16.

Brain J . Free cells in the lungs. Some aspects of their role, quantitation, and regulation. Arch Intern Med. 1970; 126(3):477-87. DOI: 10.1001/archinte.126.3.477. View

17.

Beck E, HOLT P, Nasrallah E . Effects of chrysotile and acid-treated chrysotile on macrophage cultures. Br J Ind Med. 1971; 28(2):179-85. PMC: 1009263. DOI: 10.1136/oem.28.2.179. View

18.

Beck C, Tappel A . Rat-liver lysosomal beta-glucosidase: a membrane enzyme. Biochim Biophys Acta. 1968; 151(1):159-64. DOI: 10.1016/0005-2744(68)90170-8. View

19.

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View

20.

MYRVIK Q, LEAKE E, FARISS B . Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961; 86:128-32. View