Characterization of the Subtilase Gene Family in Tomato (Lycopersicon Esculentum Mill.)

Overview

Journal Plant Mol Biol

Date 1999 Jun 1

PMID 10350089

Citations 35

Authors

J Meichtry

N Amrhein

A Schaller

Affiliations

Soon will be listed here.

Abstract

The gene family of subtilisin-like serine proteases (subtilases, SBTs) in tomato (Lycopersicon esculentum Mill.) comprises at least 15 members, 12 of which have been characterized in this study. Sequence comparison revealed that tomato subtilases fall into 5 distinct subfamilies. Single genes were shown to exist for LeSBT1, LeSBT2 and tmp), while 5 and 6 genes were found in the LeSBT3/4 and P69 subfamilies, respectively. With the exception of tmp, tomato subtilase genes were found to lack introns. Expression of subtilase genes was confirmed at the mRNA level by northern blot analysis and/or by primer extension experiments. For each of the 5 subtilase subfamilies, a distinctive pattern of expression was observed in tomato organs. At least one of the subtilases was found to be expressed in each organ analysed. Structural features evident from deduced amino acid sequences are discussed with reference to the related mammalian proprotein convertases.

Citing Articles

Genome-Wide Analysis and Characterization of the Riemerella anatipestifer Putative T9SS Secretory Proteins with a Conserved C-Terminal Domain.

Chen Z, Niu P, Ren X, Han W, Shen R, Zhu M J Bacteriol. 2022; 204(7):e0007322.

PMID: 35670588 PMC: 9295540. DOI: 10.1128/jb.00073-22.

Riemerella anatipestifer T9SS Effector SspA Functions in Bacterial Virulence and Defending Natural Host Immunity.

Chen Z, Niu P, Ren X, Han W, Shen R, Zhu M Appl Environ Microbiol. 2022; 88(11):e0240921.

PMID: 35575548 PMC: 9195944. DOI: 10.1128/aem.02409-21.

Genome-Wide Investigation of Family Genes in Pineapple and Functional Analysis of AcoSBT1.12 in Floral Transition.

Jin X, Liu Y, Hou Z, Zhang Y, Fang Y, Huang Y Front Genet. 2021; 12:730821.

PMID: 34557223 PMC: 8452990. DOI: 10.3389/fgene.2021.730821.

Bacillus amyloliquefaciens MBI600 differentially induces tomato defense signaling pathways depending on plant part and dose of application.

Dimopoulou A, Theologidis I, Liebmann B, Kalantidis K, Vassilakos N, Skandalis N Sci Rep. 2019; 9(1):19120.

PMID: 31836790 PMC: 6910970. DOI: 10.1038/s41598-019-55645-2.

Changes in the microsomal proteome of tomato fruit during ripening.

Pontiggia D, Spinelli F, Fabbri C, Licursi V, Negri R, De Lorenzo G Sci Rep. 2019; 9(1):14350.

PMID: 31586085 PMC: 6778153. DOI: 10.1038/s41598-019-50575-5.

References

Shennan K, Taylor N, Jermany J, Matthews G, Docherty K . Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events. J Biol Chem. 1995; 270(3):1402-7. DOI: 10.1074/jbc.270.3.1402. View

Seidah N, Chretien M, Day R . The family of subtilisin/kexin like pro-protein and pro-hormone convertases: divergent or shared functions. Biochimie. 1994; 76(3-4):197-209. DOI: 10.1016/0300-9084(94)90147-3. View

Uchikoba T, Yonezawa H, Kaneda M . Cleavage specificity of cucumisin, a plant serine protease. J Biochem. 1995; 117(5):1126-30. DOI: 10.1093/oxfordjournals.jbchem.a124817. View

Gilbert W, Marchionni M, McKnight G . On the antiquity of introns. Cell. 1986; 46(2):151-3. DOI: 10.1016/0092-8674(86)90730-0. View

Barr P . Mammalian subtilisins: the long-sought dibasic processing endoproteases. Cell. 1991; 66(1):1-3. DOI: 10.1016/0092-8674(91)90129-m. View

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

Royo J, Nass N, Matton D, Okamoto S, Clarke A, Newbigin E . A retrotransposon-like sequence linked to the S-locus of Nicotiana alata is expressed in styles in response to touch. Mol Gen Genet. 1996; 250(2):180-8. DOI: 10.1007/BF02174177. View

Schaller A, Ryan C . Identification of a 50-kDa systemin-binding protein in tomato plasma membranes having Kex2p-like properties. Proc Natl Acad Sci U S A. 1994; 91(25):11802-6. PMC: 45323. DOI: 10.1073/pnas.91.25.11802. View

Steiner D, Smeekens S, Ohagi S, Chan S . The new enzymology of precursor processing endoproteases. J Biol Chem. 1992; 267(33):23435-8. View

10.

Kinal H, Park C, Berry J, Koltin Y, Bruenn J . Processing and secretion of a virally encoded antifungal toxin in transgenic tobacco plants: evidence for a Kex2p pathway in plants. Plant Cell. 1995; 7(6):677-88. PMC: 160815. DOI: 10.1105/tpc.7.6.677. View

11.

Siezen R, Leunissen J . Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci. 1997; 6(3):501-23. PMC: 2143677. DOI: 10.1002/pro.5560060301. View

12.

Rose A, Last R . Introns act post-transcriptionally to increase expression of the Arabidopsis thaliana tryptophan pathway gene PAT1. Plant J. 1997; 11(3):455-64. DOI: 10.1046/j.1365-313x.1997.11030455.x. View

13.

Heitz T, Bergey D, Ryan C . A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate. Plant Physiol. 1997; 114(3):1085-93. PMC: 158398. DOI: 10.1104/pp.114.3.1085. View

14.

Creemers J, Jackson R, Hutton J . Molecular and cellular regulation of prohormone processing. Semin Cell Dev Biol. 1998; 9(1):3-10. DOI: 10.1006/scdb.1997.0195. View

15.

Tornero P, Mayda E, Gomez M, Canas L, Conejero V, Vera P . Characterization of LRP, a leucine-rich repeat (LRR) protein from tomato plants that is processed during pathogenesis. Plant J. 1996; 10(2):315-30. DOI: 10.1046/j.1365-313x.1996.10020315.x. View

16.

Taylor A, Horsch A, Rzepczyk A, Hasenkampf C, RIGGS C . Maturation and secretion of a serine proteinase is associated with events of late microsporogenesis. Plant J. 1998; 12(6):1261-71. DOI: 10.1046/j.1365-313x.1997.12061261.x. View

17.

Benner S, Badcoe I, Cohen M, Gerloff D . Bona fide prediction of aspects of protein conformation. Assigning interior and surface residues from patterns of variation and conservation in homologous protein sequences. J Mol Biol. 1994; 235(3):926-58. DOI: 10.1006/jmbi.1994.1049. View

18.

Gensberg K, Jan S, Matthews G . Subtilisin-related serine proteases in the mammalian constitutive secretory pathway. Semin Cell Dev Biol. 1998; 9(1):11-7. DOI: 10.1006/scdb.1997.0196. View

19.

Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C . Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature. 1998; 391(6666):485-8. DOI: 10.1038/35140. View

20.

Siezen R, de Vos W, Leunissen J, Dijkstra B . Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases. Protein Eng. 1991; 4(7):719-37. DOI: 10.1093/protein/4.7.719. View