Histidine Operon Deattenuation in DnaA Mutants of Salmonella Typhimurium Correlates with a Decrease in the Gene Dosage Ratio Between TRNA(His) and Histidine Biosynthetic Loci

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Apr 28

PMID 10217789

Citations 5

Authors

L Bossi

Affiliations

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Abstract

Expression of the histidine operon of Salmonella typhimurium is increased in dnaA(Ts) mutants at 37 degrees C. This effect requires an intact his attenuator and can be suppressed by increasing the gene copy number of the hisR locus, which encodes the tRNA(His). We present data which suggest that the his deattenuation defect in dnaA(Ts) mutants results from the loss of a gene dosage gradient between the hisR locus, close to oriC, and the his operon, far from oriC. Some of the conclusions drawn here may apply to other operons as well.

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