Increased Elongation of N-acetyllactosamine Repeats in Doubly Glycosylated Lysozyme with a Particular Spacing of the Glycosylation Sites

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 1999 Apr 22

PMID 10211704

Citations 1

Authors

R Melcher

H W Grosch

O Grosse

A Hasilik

Affiliations

Soon will be listed here.

Abstract

Lysozyme is an example of an extensively studied secretory enzyme. Glycosylated mutant human lysozyme has been used as a model in studies on the biosynthesis of N-acetyllactosamine repeats in N-linked oligosaccharides. We examined the biosynthesis of the repeats in two doubly glycosylated mutants and describe here a rapid purification and separation of singly and doubly glycosylated molecules. In one of the mutants, the elongation of the repeats is enhanced if the molecules are doubly glycosylated, but not if the carbohydrate is attached to either site individually. This enhancement is not seen in the other doubly glycosylated mutant. Since lysozyme is not structurally related to glycoproteins bearing carbohydrate with N-acetyllactosamine repeats, we propose that in multivalent substrates the synthesis of the repeats can be promoted by a proper spacing of the elongated carbohydrate antennae in addition to any role of the protein backbone.

Citing Articles

Glycosylation-site-selective synthesis of N-acetyl-lactosamine repeats in bis-glycosylated human lysozyme.

Melcher R, Hillebrand A, Bahr U, Schroder B, Karas M, Hasilik A Biochem J. 2000; 348 Pt 3:507-15.

PMID: 10839980 PMC: 1221091.

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