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Discrimination of Solvent from Protein Regions in Native Fouriers As a Means of Evaluating Heavy-atom Solutions in the MIR and MAD Methods

Overview
Journal Acta Crystallogr D Biol Crystallogr
Specialty Chemistry
Date 1999 Mar 25
PMID 10089362
Citations 21
Authors
T C Terwilliger
J Berendzen
Affiliations
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Abstract

An automated examination of the native Fourier is tested as a means of evaluation of a heavy-atom solution in MAD and MIR methods for macromolecular crystallography. It is found that the presence of distinct regions of high and low density variation in electron-density maps is a good indicator of the correctness of a heavy-atom solution in the MIR and MAD methods. The method can be used to evaluate heavy-atom solutions during MAD and MIR structure solutions and to determine the handedness of the structure if anomalous data have been measured.

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