Adrenergic Control of the Ultrarapid Delayed Rectifier Current in Canine Atrial Myocytes

Overview

Journal J Physiol

Specialty Physiology

Date 1999 Mar 24

PMID 10087339

Citations 8

Authors

L Yue

J Feng

Z Wang

S Nattel

Affiliations

Soon will be listed here.

Abstract

1. The effects of adrenergic stimulation on the ultrarapid delayed rectifier K+ current (IKur,d) of dog atrial myocytes was studied with patch-clamp methods. 2. Isoproterenol (isoprenaline) increased IKur,d in a concentration-dependent fashion with an EC50 of 7.3 +/- 0.8 nM. The effect of isoproterenol was blocked by propranolol, mimicked by forskolin and 8-bromo-cAMP, and prevented by inhibition of protein kinase A. 3. Phenylephrine (in the presence of propranolol) increased IKur,d with an EC50 of 0.49 +/- 0.06 microM. The effect of phenylephrine was blocked by prazosin, prevented by inhibition of protein kinase C, and mimicked by activation of protein kinase C with phorbol ester. 4. Phenylephrine significantly abbreviated canine atrial action potential duration in the absence of tetraethylammonium (TEA). When TEA was present under both control conditions and in the presence of phenylephrine, phenylephrine failed to alter canine atrial repolarization. 5. We conclude that beta- and alpha-adrenergic stimulation increase IKur,d via protein kinase A and C, respectively, and that the induced changes in IKur,d may play a role in adrenergic control of canine atrial repolarization.

Citing Articles

Ion channel macromolecular complexes in cardiomyocytes: roles in sudden cardiac death.

Abriel H, Rougier J, Jalife J Circ Res. 2015; 116(12):1971-88.

PMID: 26044251 PMC: 4471480. DOI: 10.1161/CIRCRESAHA.116.305017.

A compartmentalized mathematical model of the β1-adrenergic signaling system in mouse ventricular myocytes.

Bondarenko V PLoS One. 2014; 9(2):e89113.

PMID: 24586529 PMC: 3931689. DOI: 10.1371/journal.pone.0089113.

Anger, emotion, and arrhythmias: from brain to heart.

Taggart P, Boyett M, Logantha S, Lambiase P Front Physiol. 2011; 2:67.

PMID: 22022314 PMC: 3196868. DOI: 10.3389/fphys.2011.00067.

Can inhibition of IKur promote atrial fibrillation?.

Burashnikov A, Antzelevitch C Heart Rhythm. 2008; 5(9):1304-9.

PMID: 18774108 PMC: 2632605. DOI: 10.1016/j.hrthm.2008.05.020.

Is there a functional correlate of Kv1.5 in the ventricle of canine heart and what would it mean for the use of I(Kur) blockers?.

Wettwer E Br J Pharmacol. 2007; 152(6):835-7.

PMID: 17876301 PMC: 2078221. DOI: 10.1038/sj.bjp.0707463.

References

Noble D, Tsien R . Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres. J Physiol. 1969; 200(1):205-31. PMC: 1350425. DOI: 10.1113/jphysiol.1969.sp008689. View

Fiset C, Clark R, Larsen T, Giles W . A rapidly activating sustained K+ current modulates repolarization and excitation-contraction coupling in adult mouse ventricle. J Physiol. 1997; 504 ( Pt 3):557-63. PMC: 1159960. DOI: 10.1111/j.1469-7793.1997.557bd.x. View

Bruckner R, Mugge A, Scholz H . Existence and functional role of alpha 1-adrenoceptors in the mammalian heart. J Mol Cell Cardiol. 1985; 17(7):639-45. DOI: 10.1016/s0022-2828(85)80063-8. View

Brown A, Birnbaumer L . Direct G protein gating of ion channels. Am J Physiol. 1988; 254(3 Pt 2):H401-10. DOI: 10.1152/ajpheart.1988.254.3.H401. View

Satoh H, Hashimoto K . Effect of alpha 1-adrenoceptor stimulation with methoxamine and phenylephrine on spontaneously beating rabbit sino-atrial node cells. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337(4):415-22. DOI: 10.1007/BF00169533. View

Hescheler J, Nawrath H, Tang M, TRAUTWEIN W . Adrenoceptor-mediated changes of excitation and contraction in ventricular heart muscle from guinea-pigs and rabbits. J Physiol. 1988; 397:657-70. PMC: 1192148. DOI: 10.1113/jphysiol.1988.sp017024. View

Walsh K, Kass R . Regulation of a heart potassium channel by protein kinase A and C. Science. 1988; 242(4875):67-9. DOI: 10.1126/science.2845575. View

Apkon M, Nerbonne J . Alpha 1-adrenergic agonists selectively suppress voltage-dependent K+ current in rat ventricular myocytes. Proc Natl Acad Sci U S A. 1988; 85(22):8756-60. PMC: 282540. DOI: 10.1073/pnas.85.22.8756. View

Glass D, Cheng H, Mende-Mueller L, Reed J, Walsh D . Primary structural determinants essential for potent inhibition of cAMP-dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein. J Biol Chem. 1989; 264(15):8802-10. View

10.

Kurachi Y, Ito H, Sugimoto T, Shimizu T, Miki I, Ui M . Alpha-adrenergic activation of the muscarinic K+ channel is mediated by arachidonic acid metabolites. Pflugers Arch. 1989; 414(1):102-4. DOI: 10.1007/BF00585635. View

11.

Harvey R, Hume J . Autonomic regulation of a chloride current in heart. Science. 1989; 244(4907):983-5. DOI: 10.1126/science.2543073. View

12.

Walsh K, Begenisich T, Kass R . Beta-adrenergic modulation of cardiac ion channels. Differential temperature sensitivity of potassium and calcium currents. J Gen Physiol. 1989; 93(5):841-54. PMC: 2216233. DOI: 10.1085/jgp.93.5.841. View

13.

Dirksen R, Sheu S . Modulation of ventricular action potential by alpha 1-adrenoceptors and protein kinase C. Am J Physiol. 1990; 258(3 Pt 2):H907-11. DOI: 10.1152/ajpheart.1990.258.3.H907. View

14.

Iwaki K, Sukhatme V, Shubeita H, Chien K . Alpha- and beta-adrenergic stimulation induces distinct patterns of immediate early gene expression in neonatal rat myocardial cells. fos/jun expression is associated with sarcomere assembly; Egr-1 induction is primarily an alpha 1-mediated response. J Biol Chem. 1990; 265(23):13809-17. View

15.

Fedida D, Shimoni Y, Giles W . Alpha-adrenergic modulation of the transient outward current in rabbit atrial myocytes. J Physiol. 1990; 423:257-77. PMC: 1189756. DOI: 10.1113/jphysiol.1990.sp018021. View

16.

Sanguinetti M, Jurkiewicz N . Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents. J Gen Physiol. 1990; 96(1):195-215. PMC: 2228985. DOI: 10.1085/jgp.96.1.195. View

17.

Boyle W, Nerbonne J . A novel type of depolarization-activated K+ current in isolated adult rat atrial myocytes. Am J Physiol. 1991; 260(4 Pt 2):H1236-47. DOI: 10.1152/ajpheart.1991.260.4.H1236. View

18.

Zipes D . Sympathetic stimulation and arrhythmias. N Engl J Med. 1991; 325(9):656-7. DOI: 10.1056/NEJM199108293250911. View

19.

Apkon M, Nerbonne J . Characterization of two distinct depolarization-activated K+ currents in isolated adult rat ventricular myocytes. J Gen Physiol. 1991; 97(5):973-1011. PMC: 2216507. DOI: 10.1085/jgp.97.5.973. View

20.

Toullec D, Pianetti P, Coste H, Bellevergue P, Grand-Perret T, Ajakane M . The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem. 1991; 266(24):15771-81. View