A Model for Studying Megakaryocyte Development and Biology

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Mar 17

PMID 10077637

Citations 13

Authors

G J Murphy

A D Leavitt

Affiliations

Soon will be listed here.

Abstract

The limited current understanding of megakaryocyte-lineage development and megakaryocyte biology is in large part because of a paucity of useful systems in which to conduct experiments. To overcome this problem, we have developed a transgenic mouse that uses the GP-Ibalpha regulatory sequences to achieve megakaryocyte-lineage restricted expression of an avian retroviral receptor. Through the transgenic avian receptor, avian retroviruses can efficiently and selectively infect megakaryocyte-lineage cells in vitro and in vivo. Serial infections can be performed to introduce and express multiple genes in the same cell. We have used this system to generate and characterize a pure population of primary CD41-positive megakaryocyte progenitors.

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