The Arabidopsis Photomorphogenic Mutant Hy1 is Deficient in Phytochrome Chromophore Biosynthesis As a Result of a Mutation in a Plastid Heme Oxygenase

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1999 Mar 11

PMID 10072395

Citations 114

Authors

T Muramoto

T Kohchi

A Yokota

I Hwang

H M Goodman

Affiliations

Soon will be listed here.

Abstract

The HY1 locus of Arabidopsis is necessary for phytochrome chromophore biosynthesis and is defined by mutants that show a long hypocotyl phenotype when grown in the light. We describe here the molecular cloning of the HY1 gene by using chromosome walking and mutant complementation. The product of the HY1 gene shows significant similarity to animal heme oxygenases and contains a possible transit peptide for transport to plastids. Heme oxygenase activity was detected in the HY1 protein expressed in Escherichia coli. Heme oxygenase catalyzes the oxygenation of heme to biliverdin, an activity that is necessary for phytochrome chromophore biosynthesis. The predicted transit peptide is sufficient to transport the green fluorescent protein into chloroplasts. The accumulation of the HY1 protein in plastids was detected by using immunoblot analysis with an anti-HY1 antiserum. These results indicate that the Arabidopsis HY1 gene encodes a plastid heme oxygenase necessary for phytochrome chromophore biosynthesis.

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References

Sun J, Loehr T, Wilks A, Ortiz de Montellano P . Identification of histidine 25 as the heme ligand in human liver heme oxygenase. Biochemistry. 1994; 33(46):13734-40. DOI: 10.1021/bi00250a026. View

Ma H, Yanofsky M, Meyerowitz E . Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1990; 87(10):3821-5. PMC: 53995. DOI: 10.1073/pnas.87.10.3821. View

Bowler C, Chua N . Emerging themes of plant signal transduction. Plant Cell. 1994; 6(11):1529-41. PMC: 160541. DOI: 10.1105/tpc.6.11.1529. View

Clack T, Mathews S, Sharrock R . The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. Plant Mol Biol. 1994; 25(3):413-27. DOI: 10.1007/BF00043870. View

Rhie G, Beale S . Phycobilin biosynthesis: reductant requirements and product identification for heme oxygenase from Cyanidium caldarium. Arch Biochem Biophys. 1995; 320(1):182-94. DOI: 10.1006/abbi.1995.1358. View

Church G, Gilbert W . Genomic sequencing. Proc Natl Acad Sci U S A. 1984; 81(7):1991-5. PMC: 345422. DOI: 10.1073/pnas.81.7.1991. View

Yoshida T, Ishikawa K, Sato M . Degradation of heme by a soluble peptide of heme oxygenase obtained from rat liver microsomes by mild trypsinization. Eur J Biochem. 1991; 199(3):729-33. DOI: 10.1111/j.1432-1033.1991.tb16177.x. View

Grill E, Somerville C . Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet. 1991; 226(3):484-90. DOI: 10.1007/BF00260662. View

WARD E, Jen G . Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA. Plant Mol Biol. 1990; 14(4):561-8. DOI: 10.1007/BF00027501. View

10.

Chory J, Peto C, Ashbaugh M, Saganich R, Pratt L, Ausubel F . Different Roles for Phytochrome in Etiolated and Green Plants Deduced from Characterization of Arabidopsis thaliana Mutants. Plant Cell. 1989; 1(9):867-880. PMC: 159823. DOI: 10.1105/tpc.1.9.867. View

11.

Nam H, Giraudat J, Den Boer B, Moonan F, Loos W, Hauge B . Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989; 1(7):699-705. PMC: 159806. DOI: 10.1105/tpc.1.7.699. View

12.

von Heijne G, Steppuhn J, Herrmann R . Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem. 1989; 180(3):535-45. DOI: 10.1111/j.1432-1033.1989.tb14679.x. View

13.

Lassner M, Lardizabal K, Metz J . A jojoba beta-Ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants. Plant Cell. 1996; 8(2):281-92. PMC: 161098. DOI: 10.1105/tpc.8.2.281. View

14.

Sharrock R, Quail P . Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev. 1989; 3(11):1745-57. DOI: 10.1101/gad.3.11.1745. View

15.

Parks B, Quail P . Phytochrome-Deficient hy1 and hy2 Long Hypocotyl Mutants of Arabidopsis Are Defective in Phytochrome Chromophore Biosynthesis. Plant Cell. 1991; 3(11):1177-1186. PMC: 160084. DOI: 10.1105/tpc.3.11.1177. View

16.

Takahashi S, Wang J, Rousseau D, Ishikawa K, Yoshida T, Takeuchi N . Heme-heme oxygenase complex: structure and properties of the catalytic site from resonance Raman scattering. Biochemistry. 1994; 33(18):5531-8. DOI: 10.1021/bi00184a023. View

17.

Brownstein B, Silverman G, LITTLE R, Burke D, Korsmeyer S, Schlessinger D . Isolation of single-copy human genes from a library of yeast artificial chromosome clones. Science. 1989; 244(4910):1348-51. DOI: 10.1126/science.2544027. View

18.

Yoshida T, Biro P, Cohen T, Muller R, Shibahara S . Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem. 1988; 171(3):457-61. DOI: 10.1111/j.1432-1033.1988.tb13811.x. View

19.

Skerra A . A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene. 1994; 141(1):79-84. DOI: 10.1016/0378-1119(94)90131-7. View

20.

Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J . Engineered GFP as a vital reporter in plants. Curr Biol. 1996; 6(3):325-30. DOI: 10.1016/s0960-9822(02)00483-9. View