Determining Anomericity of the Glycosidic Bond in Zn(II)-diethylenetriamine-disaccharide Complexes Using MSn in a Quadrupole Ion Trap

Overview

Journal J Am Soc Mass Spectrom

Specialty Chemistry

Date 1999 Mar 9

PMID 10069047

Citations 14

Authors

S P Gaucher

J A Leary

Affiliations

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Abstract

Zinc-diethylenetriamine (Zn-dien) N-glycoside complexes of four 1,4 and four 1,6 linked disaccharides are prepared. Each reaction mixture is ionized by electrospray and the resulting species [Zn(dien)(disaccharide)-H]+ is allowed to undergo collision-induced dissociation in a quadrupole ion trap. An MS3 analysis is used to differentiate alpha versus beta anomericity of the glycosidic bond in the disaccharide moiety. In addition, the MS2 and MS3 spectra can be used together to determine the linkage position of this glycosidic bond.

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