UV-A-induced Decrease in Nuclear Factor-kappaB Activity in Human Keratinocytes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1999 Mar 3

PMID 10051429

Citations 7

Authors

M Djavaheri-Mergny

M P Gras

J L Mergny

L Dubertret

Affiliations

Soon will be listed here.

Abstract

Previous reports have demonstrated an increase in nuclear factor-kappaB (NF-kappaB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320-400 nm) on NF-kappaB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-kappaB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-kappaB inducers. Moreover, UV-A radiation induces a decrease in NF-kappaB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IkappaBalpha (IkappaB is the NF-kappaB inhibitor), which is closely associated with NF-kappaB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-kappaB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-kappaB activity in mammalian cells, probably through degradation of NF-kappaB protein subunits. These findings suggest that UV-A could modulate the NF-kappaB-dependent gene expression.

Citing Articles

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UVB-stimulated TNFα release from human melanocyte and melanoma cells is mediated by p38 MAPK.

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Detection of UV-induced activation of NF-kappaB in a recombinant human cell line by means of Enhanced Green Fluorescent Protein (EGFP).

Hellweg C, Baumstark-Khan C Radiat Environ Biophys. 2007; 46(3):269-79.

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The role of JNK and p38 MAPK activities in UVA-induced signaling pathways leading to AP-1 activation and c-Fos expression.

Silvers A, Bachelor M, Timothy Bowden G Neoplasia. 2003; 5(4):319-29.

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Gamma-glutamyl transpeptidase activity mediates NF-kappaB activation through lipid peroxidation in human leukemia U937 cells.

Djavaheri-Mergny M, Accaoui M, Rouillard D, Wietzerbin J Mol Cell Biochem. 2002; 232(1-2):103-11.

PMID: 12030366 DOI: 10.1023/a:1014834315936.

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