Isolation of Vibrio Vulnificus Serovar E from Aquatic Habitats in Taiwan

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1999 Mar 2

PMID 10049908

Citations 10

Authors

C Amaro

L. I. Hor

E Marco-Noales

T Bosque

B Fouz

E Alcaide

Affiliations

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Abstract

The existence of strains of Vibrio vulnificus serovar E that are avirulent for eels is reported in this work. These isolates were recovered from water and oysters and differed from eel virulent strains in (i) fermentation and utilization of mannitol, (ii) ribotyping after HindIII digestion, and (iii) susceptibility to eel serum. Lipopolysaccharide of these strains lacked the highest molecular weight immunoreactive bands, which are probably involved in serum resistance.

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References

Arias C, Verdonck L, Swings J, Garay E, Aznar R . Intraspecific Differentiation of Vibrio vulnificus Biotypes by Amplified Fragment Length Polymorphism and Ribotyping. Appl Environ Microbiol. 1997; 63(7):2600-6. PMC: 1389195. DOI: 10.1128/aem.63.7.2600-2606.1997. View

Hoi L, Larsen J, Dalsgaard I, Dalsgaard A . Occurrence of Vibrio vulnificus biotypes in Danish marine environments. Appl Environ Microbiol. 1998; 64(1):7-13. PMC: 124664. DOI: 10.1128/AEM.64.1.7-13.1998. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Amaro C, Biosca E . Vibrio vulnificus biotype 2, pathogenic for eels, is also an opportunistic pathogen for humans. Appl Environ Microbiol. 1996; 62(4):1454-7. PMC: 167917. DOI: 10.1128/aem.62.4.1454-1457.1996. View

Fouz B, Mazoy R, Lemos M, del Olmo M, Amaro C . Utilization of hemin and hemoglobin by Vibrio vulnificus biotype 2. Appl Environ Microbiol. 1996; 62(8):2806-10. PMC: 168066. DOI: 10.1128/aem.62.8.2806-2810.1996. View

Biosca E, Oliver J, Amaro C . Phenotypic characterization of Vibrio vulnificus biotype 2, a lipopolysaccharide-based homogeneous O serogroup within Vibrio vulnificus. Appl Environ Microbiol. 1996; 62(3):918-27. PMC: 167856. DOI: 10.1128/aem.62.3.918-927.1996. View

Biosca E, Amaro C . Toxic and enzymatic activities of Vibrio vulnificus biotype 2 with respect to host specificity. Appl Environ Microbiol. 1996; 62(7):2331-7. PMC: 168013. DOI: 10.1128/aem.62.7.2331-2337.1996. View

Merino S, Alberti S, Tomas J . Aeromonas salmonicida resistance to complement-mediated killing. Infect Immun. 1994; 62(12):5483-90. PMC: 303292. DOI: 10.1128/iai.62.12.5483-5490.1994. View

Tison D, Nishibuchi M, Greenwood J, Seidler R . Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol. 1982; 44(3):640-6. PMC: 242070. DOI: 10.1128/aem.44.3.640-646.1982. View

10.

Wan L . Isolation and Characterization of Vibrio vulnificus Inhabiting the Marine Environment of the Southwestern Area of Taiwan. J Biomed Sci. 1995; 2(4):384-389. DOI: 10.1007/BF02255226. View

11.

Amaro C, Fouz B, Biosca E, Marco-Noales E, Collado R . The lipopolysaccharide O side chain of Vibrio vulnificus serogroup E is a virulence determinant for eels. Infect Immun. 1997; 65(6):2475-9. PMC: 175347. DOI: 10.1128/iai.65.6.2475-2479.1997. View

12.

Amaro C, Biosca E, Fouz B, Alcaide E, Esteve C . Evidence that water transmits Vibrio vulnificus biotype 2 infections to eels. Appl Environ Microbiol. 1995; 61(3):1133-7. PMC: 167367. DOI: 10.1128/aem.61.3.1133-1137.1995. View

13.

Biosca E, Amaro C, Larsen J, Pedersen K . Phenotypic and genotypic characterization of Vibrio vulnificus: proposal for the substitution of the subspecific taxon biotype for serovar. Appl Environ Microbiol. 1997; 63(4):1460-6. PMC: 168441. DOI: 10.1128/aem.63.4.1460-1466.1997. View

14.

Amaro C, Biosca E, Fouz B, Toranzo A, Garay E . Role of iron, capsule, and toxins in the pathogenicity of Vibrio vulnificus biotype 2 for mice. Infect Immun. 1994; 62(2):759-63. PMC: 186176. DOI: 10.1128/iai.62.2.759-763.1994. View

15.

Biosca E, Marco-Noales E, Amaro C, Alcaide E . An enzyme-linked immunosorbent assay for detection of Vibrio vulnificus biotype 2: development and field studies. Appl Environ Microbiol. 1997; 63(2):537-42. PMC: 168346. DOI: 10.1128/aem.63.2.537-542.1997. View

16.

Joiner K, Grossman N, Schmetz M, Leive L . C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella montevideo. J Immunol. 1986; 136(2):710-5. View