Formation of Knots in Partially Replicated DNA Molecules

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 1999 Feb 20

PMID 10024438

Citations 40

Authors

J M Sogo

A Stasiak

M L Martinez-Robles

D B Krimer

P Hernandez

J B Schvartzman

Affiliations

Soon will be listed here.

Abstract

Bacterial plasmids with two origins of replication in convergent orientation are frequently knotted in vivo. The knots formed are localised within the newly replicated DNA regions. Here, we analyse DNA knots tied within replication bubbles of such plasmids, and observe that the knots formed show predominantly positive signs of crossings. We propose that helical winding of replication bubbles in vivo leads to topoisomerase-mediated formation of knots on partially replicated DNA molecules.

Citing Articles

Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules.

Cebrian J, Martinez V, Hernandez P, Krimer D, Martinez-Robles M, Schvartzman J Bio Protoc. 2024; 14(9):e4983.

PMID: 38737504 PMC: 11082789. DOI: 10.21769/BioProtoc.4983.

Knot Formation on DNA Pushed Inside Chiral Nanochannels.

Ruskova R, Racko D Polymers (Basel). 2023; 15(20).

PMID: 37896430 PMC: 10611388. DOI: 10.3390/polym15204185.

Topological gelation of reconnecting polymers.

Bonato A, Marenduzzo D, Michieletto D, Orlandini E Proc Natl Acad Sci U S A. 2022; 119(44):e2207728119.

PMID: 36279471 PMC: 9636914. DOI: 10.1073/pnas.2207728119.

Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.

Cebrian J, Martinez V, Hernandez P, Krimer D, Fernandez-Nestosa M, Schvartzman J Biology (Basel). 2021; 10(11).

PMID: 34827187 PMC: 8615216. DOI: 10.3390/biology10111195.

Channels with Helical Modulation Display Stereospecific Sensitivity for Chiral Superstructures.

Ruskova R, Racko D Polymers (Basel). 2021; 13(21).

PMID: 34771282 PMC: 8588256. DOI: 10.3390/polym13213726.